539166 Anti-19S Proteasome S2-Subunit Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H Rb Polyclonal Antibody

Pricing & Availability

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539166-100UL
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      Glass bottle 100 ul
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      Description
      OverviewRecognizes the ~100 kDa 19S proteasome S2-subunit in HeLa cells and partially purified 26S proteasome preparations. Also recognizes an ~50 kDa potential subunit degradation product.
      Catalogue Number539166
      Brand Family Calbiochem®
      Application Data
      Detection of human 19S proteasome S2-subunit by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-19S Proteasome S2-Subunit Rabbit pAb (Cat. No. 539166) (1:1000). Detection: chemiluminescence.
      References
      ReferencesKloetzel, P.M. 2001. Nat. Rev. Mol. Cell Biol. 2, 179.
      Yew, P.R. 2001. J. Cell Physiol. 187, 1.
      Chaturvedi, K., et al. 2000. Biopolymers 55, 334.
      Orlowski, M., and Wilk, S. 2000. Arch. Biochem. Biophys. 383, 1.
      Pickart, C.M. 2000. Trends Biochem Sci. 25, 544.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Positive controlHeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Immunoprecipitation (see comments)
      Application CommentsAlso detects an ~50 kDa band, presumably corresponding to a subunit degradation product. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenrecombinant, human 19S proteasome S2-subunit
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-19S Proteasome S2-Subunit Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-19S Proteasome S2-Subunit Rabbit pAb Certificates of Analysis

      TitleLot Number
      539166

      References

      Reference overview
      Kloetzel, P.M. 2001. Nat. Rev. Mol. Cell Biol. 2, 179.
      Yew, P.R. 2001. J. Cell Physiol. 187, 1.
      Chaturvedi, K., et al. 2000. Biopolymers 55, 334.
      Orlowski, M., and Wilk, S. 2000. Arch. Biochem. Biophys. 383, 1.
      Pickart, C.M. 2000. Trends Biochem Sci. 25, 544.

      Citations

      Title
    • Emily I. Chen, et al. (2005) Maspin alters the carcinoma proteome. FASEB Journal in press,.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-August-2007 RFH
      ApplicationImmunoblotting (1:1000)
      Immunoprecipitation (see comments)
      Application Data
      Detection of human 19S proteasome S2-subunit by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-19S Proteasome S2-Subunit Rabbit pAb (Cat. No. 539166) (1:1000). Detection: chemiluminescence.
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~100 kDa 19S proteasome S2-subunit protein.
      BackgroundProteolytic degradation is an important regulatory component of various cell processes including metabolism, stress response, antigen presentation, cell cycle regulation and modulation of cell surface receptors. The ubiquitin-proteasome pathway is the predominant mode of this proteolytic degradation. The 26S proteasome is an ATP-dependent multisubunit (~31 subunits), barrel shaped molecular machine with an apparent molecular weight of ~2.5 Mda. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. A series of enzymes is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Defects in the ubiquitin-proteasome pathway are associated with several genetic diseases including cystic fibrosis and Angelman's syndrome.
      HostRabbit
      Immunogen speciesHuman
      Immunogenrecombinant, human 19S proteasome S2-subunit
      IsotypeIgG
      Specieshuman
      Positive controlHeLa cells
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAlso detects an ~50 kDa band, presumably corresponding to a subunit degradation product. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKloetzel, P.M. 2001. Nat. Rev. Mol. Cell Biol. 2, 179.
      Yew, P.R. 2001. J. Cell Physiol. 187, 1.
      Chaturvedi, K., et al. 2000. Biopolymers 55, 334.
      Orlowski, M., and Wilk, S. 2000. Arch. Biochem. Biophys. 383, 1.
      Pickart, C.M. 2000. Trends Biochem Sci. 25, 544.
      Citation
    • Emily I. Chen, et al. (2005) Maspin alters the carcinoma proteome. FASEB Journal in press,.