Millipore Sigma Vibrant Logo

AB9732 Anti-14-3-3 ε Antibody, CT

View Products on Sigmaaldrich.com
AB9732
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, R, Rb, ShELISA, WB, IHCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB9732
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-14-3-3 ε Antibody, CT
      Background InformationMammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.

      The 14-3-3 epsilon, C-terminal antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's Disease.
      References
      Product Information
      FormatSerum
      PresentationLiquid.
      Quality LevelMQ100
      Applications
      ApplicationAnti-14-3-3 ε Antibody, C-terminal is an antibody against 14-3-3 ε for use in ELISA, WB, IH.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 1:3,000 using ECL.
      Immunohistochemistry
      ELISA
      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide from the C-terminal area of 14-3-3 epsilon.
      HostRabbit
      Specificity14-3-3 epsilon, C-terminal. By Western blot the antibody recognizes the ~33 kDa protein.
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Sheep
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.
      Gene Symbol
      • YWHAE
      • KCIP-1
      • MDS
      • 14-3-3E
      • MDCR
      • FLJ45465
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62258 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
      SIZE: 255 amino acids; 29174 Da
      SUBUNIT: Homodimer. Interacts with NDEL1 (By similarity). Interacts with HCV core protein.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      SIMILARITY: SwissProt: P62258 ## Belongs to the 14-3-3 family.
      Molecular Weight33 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

      During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-14-3-3 ε Antibody, CT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-14-3-3 ε Antibody, CT Certificates of Analysis

      TitleLot Number
      Anti-14-3-3 epsilon, C-terminal - 3277977 3277977
      Anti-14-3-3 epsilon, C-terminal - 3508315 3508315
      Anti-14-3-3 epsilon, C-terminal -2535766 2535766

      References

      Reference overviewPub Med ID
      Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein.
      Steinacker, Petra, et al.
      Mol. Cell. Biol., 25: 1339-46 (2005)  2005

      Show Abstract
      15684385 15684385
      Mammalian and yeast 14-3-3 isoforms form distinct patterns of dimers in vivo
      Chaudhri, Maliha, et al
      Biochem Biophys Res Commun, 300:679-85 (2003)  2003

      12507503 12507503
      Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease
      Wiltfang, J, et al
      J Neurochem, 73:2485-90 (1999)  1999

      10582609 10582609
      Expression and structural analysis of 14-3-3 proteins
      Jones, D H, et al
      J Mol Biol, 245:375-84 (1995)  1995

      7837270 7837270

      Related Products & Applications

      Related Products

      Catalog Number Description  
      AB9734 Anti-14-3-3 ε Antibody, NT Show Pricing & Availability
      AB9750 Anti-14-3-3 phospho Serine58 Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies