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MAB3478 Anti-1-cys Peroxiredoxin Antibody, clone 8H11

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MAB3478
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, ICC, IHCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB3478
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-1-cys Peroxiredoxin Antibody, clone 8H11
      Alternate Names
      • aiPLA2
      • Peroxiredoxin 6
      • Non-selenium Glutathione Peroxidase
      Background Information1-Cys-Peroxiredoxin is a cytosolic protein that is involved in the regulation of cellular redox potential. 1-Cys peroxiredoxin is a bifunctional enzyme with glutathione peroxidase and phospholipase A2 activities (Chen, 2000). It can reduce hydrogen peroxide, short chain fatty acids, and phospholipid hydroperoxides and is believed to play a role in phospholipid turnover as well as in the protection against oxidative injury.
      References
      Product Information
      FormatAscites
      PresentationAscites fluid. Liquid.
      Quality LevelMQ100
      Applications
      ApplicationDetect 1-cys Peroxiredoxin using this Anti-1-cys Peroxiredoxin Antibody, clone 8H11 validated for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern blot on rat lung lysate (24 kDa) or recombinant human protein.

      Immunohistochemistry on 4% paraformaldehyde fixed rat lung tissue.

      Immunocytochemistry on rat type II alveolar epithelial cells.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant human 1-Cys peroxiredoxin.
      Clone8H11
      HostMouse
      Specificity1-Cys-Peroxiredoxin (Prx VI).
      IsotypeIgM
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
      Gene Symbol
      • PRDX6
      • p29
      • Peroxiredoxin-6
      • MGC46173
      • PRX
      • aiPLA2
      • AOP2
      • 1-Cys
      • KIAA0106
      • NSGPx
      • EC 1.11.1.15
      • EC 3.1.1.-
      • EC 1.11.1.7
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P30041 # Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
      SIZE: 224 amino acids; 25035 Da
      SUBUNIT: Homotetramer. May interact with HTR2A (By similarity). Interacts with STH.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Lysosome (By similarity). Cytoplasmic vesicle (By similarity). Note=And also found in lung secretory organelles (By similarity).
      SIMILARITY: SwissProt: P30041 ## Belongs to the ahpC/TSA family. Rehydrin subfamily.
      MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin. & Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-1-cys Peroxiredoxin Antibody, clone 8H11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-1-cys Peroxiredoxin Antibody, clone 8H11 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-1-CYS PEROXIREDOXIN MONOCLONAL ANTIBODY - 2110501 2110501
      MOUSE ANTI-1-CYS PEROXIREDOXIN - 3284417 3284417
      MOUSE ANTI-1-CYS PEROXIREDOXIN -2662374 2662374
      MOUSE ANTI-1-CYS PEROXIREDOXIN -2669984 2669984
      MOUSE ANTI-1-CYS PEROXIREDOXIN -2680724 2680724
      MOUSE ANTI-1-CYS PEROXIREDOXIN -2696607 2696607
      MOUSE ANTI-1-CYS PEROXIREDOXIN -2785808 2785808
      MOUSE ANTI-1-CYS PEROXIREDOXIN MONOCLONAL ANTIBODY - 2095339 2095339
      MOUSE ANTI-1-CYS PEROXIREDOXIN MONOCLONAL ANTIBODY - 2151854 2151854

      References

      Reference overviewPub Med ID
      Lysosomal-type PLA2 and turnover of alveolar DPPC.
      Fisher, A B and Dodia, C
      Am. J. Physiol. Lung Cell Mol. Physiol., 280: L748-54 (2001)  2001

      Show Abstract
      11238016 11238016