164598 Aminopeptidase M, Porcine Kidney

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164598
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Overview

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164598-50U
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      Description
      OverviewNative amino peptidase M from porcine kidney. Metalloprotease that hydrolyzes N-terminal amino acids from almost all unsubstituted oligopeptides. Does not cleave X-Pro bonds or N-blocked amino acids. Useful in peptide sequencing. Inhibited by EDTA, bestatin, and amastatin.
      Catalogue Number164598
      Brand Family Calbiochem®
      SynonymsAminopeptidase M
      References
      ReferencesWachsmuth, E.E., et al. 1966. Biochemistry 5, 169.
      Product Information
      CAS number9054-63-1
      Activity≥50 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-<i>p</i>NA per min at 37°C, pH 7.2.
      EC number3.4.11.2
      FormWhite crystalline suspension
      FormulationCrystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥10 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsOxidases: ≤3%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38-42

      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Aminopeptidase M, Porcine Kidney SDS

      Title

      Safety Data Sheet (SDS) 

      Aminopeptidase M, Porcine Kidney Certificates of Analysis

      TitleLot Number
      164598

      References

      Reference overview
      Wachsmuth, E.E., et al. 1966. Biochemistry 5, 169.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-April-2011 JSW
      SynonymsAminopeptidase M
      DescriptionNative amino peptidase M from porcine kidney. Metalloprotease that hydrolyzes N-terminal amino acids from almost all unsubstituted oligopeptides. Does not cleave X-Pro bonds or N-blocked amino acids. Useful in peptide sequencing. Inhibited by EDTA, bestatin, and amastatin.
      FormWhite crystalline suspension
      FormulationCrystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      CAS number9054-63-1
      EC number3.4.11.2
      ContaminantsOxidases: ≤3%
      Specific activity≥10 units/mg protein
      Activity≥50 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-pNA per min at 37°C, pH 7.2.
      SolubilityH₂O or physiological buffers
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
      Toxicity Harmful
      ReferencesWachsmuth, E.E., et al. 1966. Biochemistry 5, 169.

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      Categories

      Life Science Research > Proteins and Enzymes > Other Enzymes