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101167 Amido black 10 B (C.I.20470)

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Key Specifications Table

CAS #EC NumberHill FormulaMolar Mass
1064-48-8213-903-1C₂₂H₁₄N₈Na₂O₉S₂616.5 g/mol

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      Glass bottle 25 g
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      Catalogue Number101167
      SynonymsBlack acid 1, Amido black 10 B (C.I. 20470), Naphthol blue black
      DescriptionAmido black 10 B (C.I.20470)
      OverviewAmido black 10 B (C.I.20470) for electrophoresis, is used for staining connective tissue, in electrophoresis, and as a redox indicator. Proteins possess free amino and carboxyl groups and are charged positively or negatively in aqueous solutions, depending on the pH. In an alkaline milieu they are negatively charged and migrate in the electric field towards the anode at a speed that depends on the number of charges they carry. This difference in migration velocities makes it possible to separate the serum protein mixture into groups of individual components.The separated protein fractions are stained with amido black and measured photometrically or densitometrically. As a rule, five fractions are observed after staining - albumin, alpha 1- and alpha 2-globulins, beta 1- and beta 2-globulins, and beta-globulins - where albumin has the highest migration velocity and gamma-globulin the lowest. Differentiation of serum proteins by electrophoresis is important for the diagnosis and prognosis of numerous diseases. Changes in protein composition can be found e. g. in inflammatory diseases, tumors, liver diseases, antibody deficiency syndromes or disturbances in protein balance. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      Product Information
      CAS number1064-48-8
      EC number213-903-1
      Hill FormulaC₂₂H₁₄N₈Na₂O₉S₂
      Molar Mass616.5 g/mol
      HS Code3204 12 00
      Structure formula ImageStructure formula Image
      Quality LevelMQ400
      Applicationfor electrophoresis
      Biological Information
      Physicochemical Information
      pH value9.0 (10 g/l, H₂O, 25 °C)
      Bulk density670 kg/m3
      Solubility10 g/l
      Materials Information
      Toxicological Information
      LD 50 oralLD50 Rat > 5000 mg/kg
      Safety Information according to GHS
      Storage class10 - 13 Other liquids and solids
      WGKWGK 3 highly hazardous to water
      Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +2°C to +30°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Dye content (spectrophotometrically)≥ 80 %
      - sodium

      passes test
      - UV/VIS-Spectrumpasses test
      Absorption maximum λ max (water)617 - 620 nm
      Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; water)750 - 960
      TLC-Testpasses test
      Loss on drying (110 °C)≤ 10 %
      Suitability for electrophoresispasses test
      Suitability as redox indicatorpasses test