Key Specifications Table
|Key Applications||NMWL||Volume||Minimum Final Concentrate Volume|
|PExt, Protein Purification||100 KDa||4 mL||50 µL|
|Color Code||Clear with pink cap|
|Application||Ultracel-100 regenerated cellulose membrane, 4 mL sample volume|
|Centrifugal Application||Protein Concentration and Desalting|
|Length||124 mm (4.88 in.)|
|Filtration Area||3 cm²|
|Minimum Final Concentrate Volume||50 µL|
|Support Material||Regenerated cellulose|
|Safety Information according to GHS|
|Product Usage Statements|
|Storage and Shipping Information|
Amicon® Ultra-4 Centrifugal Filter Unit SDS
|Reference overview||Application||Pub Med ID|
|Labeling of biotin antibodies with horseradish peroxidase using cyanuric chloride.|
Abuknesha, Ramadan A, et al.
Nat Protoc, 4: 452-60 (2009) 2009
In this report, we describe a two-step protocol for labeling of an affinity-purified antibody to biotin with horseradish peroxidase (HRP) using cyanuric chloride (CC) as a bridge. The enzyme was first modified with CC, and following chromatography on a PD-10 column, the activated HRP was incubated with the antibody to effect coupling of the two proteins. Assessment of the conjugate product was carried out using ELISA and SDS-PAGE electrophoresis where evidence for high antibody activity, high specific activity of the conjugate preparation, coupling of nearly all the antibody and over 90% of the enzyme was shown. The titer of the conjugate exceeded 1/100,000. High molecular weight complexes were observed in the SDS-PAGE results, indicating an efficient conjugation procedure. The presence of high molecular weight complexes indicated an efficient conjugation procedure. The protocol is simple, and the conjugation steps can be completed in 27 h once the preparatory phase has been carried out; the method is entirely generic and may be applied to labeling of any antibody.
|Modification of Allergenicity and immunogenicity of formate dehydrogenase by conjugation with linear mono methoxy poly ethylene glycol: Improvement in detoxification of formate in methanol poisoning|
Muthuvel A., Rajamani R., Senthilvelan M., Manikandan s., Sheeladevi R.
Clinica Chimica acta 374 (2006) 122-128 2006
|REFINE PROTEIN PREPARATION. Tools for better protein analysis|
|Amicon® Ultra-4 Centrifugal Filter Devices User Guide|