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SCR004 Alkaline Phosphatase Detection Kit

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SCR004
100 assays  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCR004
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAlkaline Phosphatase Detection Kit
      OverviewMillipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
      Materials Required but Not Delivered1. Fixative (e.g. 4% Paraformaldehyde in PBS)

      2. 1 x Rinse Buffer (e.g. TBST: 20mM Tris-HCl, pH 7.4, 0.15M NaCl, 0.05% Tween-20)

      3. Hematoxylin (optional)

      4. Microscope
      References
      Product Information
      Components
      • Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.
      • Napthol AS-BI phosphate solution (4mg/mL) in AMPD buffer (2mol/L), pH 9.5 (Part No. 90234). One 15mL bottle.
      Quality LevelMQ100
      Applications
      ApplicationThis Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.
      Key Applications
      • Stem Cell Culture
      Application NotesEmbryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore's proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4.

      Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

      Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].

      For Research Use Only; Not for use in diagnostic procedures
      Biological Information
      Species Reactivity
      • Mammals
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Cardiac Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity.

      When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

      Alkaline Phosphatase Detection Kit SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      Murine Embryonic Stem Cell Culture

      Technical Info

      Title
      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      A Novel Serum-free and Xeno-free Differentiation Medium for Accelerated Osteogenic Differentiation of Human Mesenchymal Stem Cells
      Accumax Dissociation Reagent for the Serial Passaging of Human Embryonic Stem Cells
      Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO Medium
      Millipore Tools for Characterizing Induced Pluripotent Stem Cells
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

      Posters

      Title
      Poster: A Novel Serum-Free and Animal Component-Free Culture Medium for the Propagation and Maintenance of Human Embryonic Stem Cells