17-10504 AccuChIP™ Unmodified-Histone H3 (Lys4) Chromatin Immunoprecipitation Internal Control

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17-10504
100 reactions  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      ChIP, ChIP-seq
      Description
      Catalogue Number17-10504
      Brand Family Upstate
      DescriptionAccuChIP™ Unmodified-Histone H3 (Lys4) Chromatin Immunoprecipitation Internal Control
      OverviewAccuChIP™ Chromatin Immunoprecipitation Internal Controls are designed to improve the accuracy of ChIP experiments. This simple to spike-in reagent is composed of a target-specific peptide sequence covalently bound to a uniquely designed double stranded nucleic acid sequence. Because AccuChIP controls mimic the behavior of cross-linked chromatin, by simply adding a known amount of the AccuChIP reagent to your chromatin and performing a standard ChIP reaction followed qPCR detection you can improve the accuracy and reproducibility of your ChIP experiments.

      Features and Advantages
      • Simple to use - just add to chromatin either before or after sonication
      • Mimics the behavior of cross-linked chromatin to allow normalization of ChIP signals
      • Target specific control - uses the same antibody used for your ChIP experiment
      • Reduces ChIP variation due to variables such as chromatin preparation, antibody affinity, inconsistent background signals from carry over, and loss of material during handling
      • Easily incorporated into virtually any ChIP protocol
      • Readily detected by qPCR
      • Easily incorporated into ChIP-seq libraries
      • Variety of uniquely designed controls available -add multiple controls to be added to the same chromatin as an process antibody specificity
      Materials Required but Not Delivered
      • Reagents and materials for chromatin isolation from cells or tissues (e.g PureEpi Chromatin Preparation and Optimization kit; EMD Millipore Cat# 17-10082)
      • Sonication device or method to shear chromatin
      • Beads, reagents and buffers to perform chromatin immunoprecipitation (e.g. Magna ChIP HiSens Chromatin Immunoprecipitation Kit EMD Millipore Cat# 17-10460, or EZ-Magna ChIP HiSens Chromatin Immunoprecipitation Kit Cat# 17-10461)
      • ChIP validated antibody or ChIPAb+™ kit
      • IgG negative control antibody (visit www.emdmillipore.com/antibodies to order the IgG negative control antibody used with this control)
      • Real time thermal cycler and qPCR reagents and primers for locus of interest
      • Reagents and instrument for next generation sequencing analysis (optional)
      References
      Product Information
      Components
      • AccuChIP™ Unmodified H3K4 Spike-In Reagent (50 pg/uL )
      • AccuChIP™ Unmodified H3K4 Detection Primer Mix (5 μM)
      • AccuChIP™ Dilution Buffer
      PresentationOne box containing three vials (one vial each of target specific AccuChIP™ Spike-In Reagent, target specific AccuChIP™ Detection Primer Mix, and AccuChIP™ Dilution Buffer)
      Quality LevelMQ100
      Applications
      ApplicationTarget-specific spike-in controls that make ChIP experiments more quantitative and accurate. Applications include:
      • Normalization of ChIP signals
      • Normalization of ChIP-seq signals
      • Analysis of antibody specificity
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAccuChIP™ Chromatin Immunoprecipitation Internal Controls are stable for 6 months at -20 ºC from date of receipt.
      AccuChIP Spike In Reagent is provided as a 20X stock and should be diluted with the provided AccuChIP™ Dilution Buffer to create a working stock. Diluted AccuChIP Spike In Reagent can be stored in aliquots at -20 ºC for up to 3 months. AccuChIP Spike In reagent is composed of a peptide-double stranded DNA conjugate in a buffer containing BSA and glycerol to reduce adherence to the tube. The use of siliconized microfuge tubes for storage of diluted material is recommended.
      Packaging Information
      Material Size100 reactions
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      AccuChIP™ Unmodified-Histone H3 (Lys4) Chromatin Immunoprecipitation Internal Control SDS

      Title

      Safety Data Sheet (SDS) 

      AccuChIP™ Unmodified-Histone H3 (Lys4) Chromatin Immunoprecipitation Internal Control Certificates of Analysis

      TitleLot Number
      AccuChIP™ Unmodified-Histone H3 (Lys4) Chromatin Immunoprecipitation Internal Control - Q2495399Q2495399

      Posters

      Title
      The use of a synthetic histonepeptide-DNA conjugates as a spike-in reagent to normalize chromatin immunoprecipitation experiments (EMD)