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15-116 ATM/Chk2 Pathway Explorer Antibody MiniPack

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, RWB, ICC, IPMUnconjugatedMonoclonal Antibody
      Catalogue Number15-116
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionATM/Chk2 Pathway Explorer Antibody MiniPack
      Alternate Names
      • A-T
      • mutated
      • AT mutated
      • TEL1
      • telomere maintenance 1, homolog
      • ataxia telangiectasia mutated
      • ataxia telangiectasia mutated (includes complementation groups A, C
        and D)
      • ataxia telangiectasia mutated protein
      • human phosphatidylinositol 3-kinase homolog
      • serine-protein kinase ATM
      • choline kinase
      • choline kinase alpha
      Background InformationPathway Explorer Antibody MiniPack:
      Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).

      Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. The essential requirement for the substrates of ATM/ATR is S/TQ. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S/TQ are negative determinants for substrate phosphorylation. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.

      The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.

      * See full size versions for corresponding references.
      Product Information
      • 05-513SP Anti-ATM, clone AM9
      • 05-740SP Anti-phospho-ATM (Ser1981),
        clone 10H11.E12
      • 05-649SP Anti-Chk2, clone 7
      • For 05-649SP use Jurkat cell lysate, HeLa cell lysate.
      • For 05-740SP use Irradiated HeLa cell lysates.
      • For 05-513SP use Raji cells
      Presentation3 individual tubes containing either Anti-ATM, clone AM9; Anti-phospho-ATM (Ser1981), clone 10H11.E12; or Anti-Chk2, clone 7
      PropertiesEach vial is 30% the size of the parent catalog number
      Quality LevelMQ100
      ApplicationThis Antibody pack contains Anti-ATM Antibody, Anti-phospho-ATM Antibody (Ser1981), Anti-Chk2 Antibody validated for use in WB, ICC & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Biological Information
      Immunogen05-649SP: Mixture of two GST fusion proteins corresponding to residues 1-223 and 217-543 of human Chk2;05-740SP: KLH-conjugated, synthetic peptide corresponding to amino acids 1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM. The immunizing sequence has 11/12 identical amino acids in mouse and rat.;05-513SP: Full length human ATM. Clone AM9.
      CloneAM9; 10H11.E12; 7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Rat
      • Mouse
      • Rat
      Species Reactivity Note05-649SP and 05-740SP: Human and mouse. Others not tested. Predicted to cross-react with rat based on sequence homology.
      05-513SP: Human. Other species not tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryATM:The protein encoded by this gene belongs to the PI3/PI4-kinase family. It is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.

      Chk2:In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • AT1
      • ATA
      • ATC
      • ATD
      • ATDC
      • ATE
      • DKFZp781A0353
      • EC
      • MGC74674
      • TEL1
      • TELO1
      • CHETK-alpha
      • CHK
      • CK
      • CKI
      • EC
      UniProt Number
      UniProt SummaryFUNCTION: Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      COFACTOR: Magnesium.
      ENZYME REGULATION: Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation.
      TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
      DISEASE: Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
      DISEASE: Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807].
      DISEASE: Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].
      SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
      Molecular Weight05-649SP: 67 kDa;05-740SP:~370 kDa;05-513SP: 350 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance05-649SP: Routinely evaluated by immunoblot on RIPA lysates from Jurkat cells, HeLa, 3T3/A31, or A431 cells.

      Western Blot Analysis: 0.1-1 μg/mL of this lot detected Chk2 in RIPA lysates from Jurkat cells. A previous lot detected Chk2 in lysates from HeLa, 3T3/A31, and A431 cells.

      05-740SP: Routinely evaluated by immunoblot on in crude lysates from irradiated HeLa cells.

      Western Blot Analysis: 0.5 µg/mL of this lot detected phosphorylated ATM in crude lysates from irradiated HeLa cells.

      05-513SP: Routinely evaluated by immunoblot on nuclear extract from Raji cells.

      Western Blot Analysis:
      1:250-1:1000 dilution of this lot detected ATM in a nuclear extract from Raji cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size3 vials/Pk
      Transport Information
      Supplemental Information



      Reference overviewPub Med ID
      Heparin-coated superparamagnetic nanoparticle-mediated adeno-associated virus delivery for enhancing cellular transduction.
      Jun-Ho Hwang,Slgirim Lee,Eunmi Kim,Jung-Suk Kim,Chang-Ha Lee,Ik-Sung Ahn,Jae-Hyung Jang
      International journal of pharmaceutics  421  2011

      Show Abstract
      22016032 22016032

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