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132450 AFB staining kit for histology

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      Catalogue Number132450
      DescriptionAFB staining kit for histology
      OverviewThe AFB staining kit for histology - for the detection of acid-fast bacteria in histological tissue, is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is a ready-to-use staining kit that when used together with other in vitro diagnostic products from our portfolio makes bacterial target structures evaluable for diagnostic purposes in histological specimen materials, for example, the lung. The AFB staining kit is based on the Ziehl-Neelsen staining method for the detection of acid-fast bacteria. The set is modified such, that heating of the carbolfuchsin solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided. The use of light green solution renders the background colour less dominant, making the acid-fast bacteria considerably more visible and thus enhancing the quality of the diagnosis. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. The AFB staining kit for histology offers a modified carbol fuchsin solution which abolishes heating (Kinyoun method). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye.
      The package is sufficient for approx. 230 applications. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.
      Product Information
      HS Code3212 90 00
      Quality LevelMQ400
      Applicationfor the detection of acid-fast bacteria in histological tissue
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      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
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      Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
      P273: Avoid release to the environment.
      P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
      P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
      P304 + P340 + P310: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
      Signal WordDanger
      Storage class3 Flammable liquids
      WGKWGK 3 highly hazardous to water
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      StorageStore at +15°C to +25°C.
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      Transport Information
      Declaration (railroad and road) ADR, RIDUN 3316 , 9, II
      Declaration (transport by air) IATA-DGRUN 3316 , 9, II
      Declaration (transport by sea) IMDG-CodeUN 3316 , 9, II
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