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116450 AFB-Color staining kit

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      Catalogue Number116450
      DescriptionAFB-Color staining kit
      OverviewThe AFB-Color staining kit - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining), is a ready-to-use solution used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin, for example smears of enriched bacterial cultures. The AFB-Color staining set is based on the Ziehl-Neelsen staining method for the detection of acid-fast bacteria. The set is modified such, that heating of the carbolfuchsin solution is dispensable. Consequently, the release of potentially hazardous phenolic vapors will be avoided.The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. The AFB-Color Kit offers a modified AFB-Color Carbol fuchsin solution which abolishes heating (Kinyoun method, cold staining).Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye.
      Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. The package is sufficient for up to 250 applications.This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      Product Information
      HS Code3212 90 00
      Quality LevelMQ400
      Applicationfor the microscopic investigation of acid-fast bacteria (cold staining)
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Pictogram(s)
      Precautionary Statement(s)P273: Avoid release to the environment.
      P301 + P330 + P331: IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
      Signal WordDanger
      Storage class3 Flammable liquids
      Safety Information
      Hazard Symbols Hazard SymbolsHarmful
      Hazard SymbolsCorrosive
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +15°C to +25°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDUN 1992 Entzündbarer flüssiger Stoff, giftig, n.a.g.(PHENOL UND ENTH. ETHANOL), 3 (6.1), II
      Declaration (transport by air) IATA-DGRUN 1992 FLAMMABLE LIQUID, TOXIC, N.O.S.(PHENOL AND CONT. ETHANOL), 3 (6.1), II
      Declaration (transport by sea) IMDG-CodeUN 1992 FLAMMABLE LIQUID, TOXIC, N.O.S.(PHENOL AND CONT. ETHANOL), 3 (6.1), II
      Supplemental Information
      Suitability for microscopypasses test


      AFB-Color staining kit SDS


      Safety Data Sheet (SDS) 

      AFB-Color staining kit Certificates of Analysis

      Product Number PackagingSpecificationLot Number
      1164500007 Fibre cartonPDF Specification Document


      Fast and reliable - All you need for histology

      Instructions for Use

      116450 AFB-Color staining kit - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining)
      116450 AFB-Color staining kit - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining) (US version)

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