437996 5-Lipoxygenase, Human, Recombinant, S. fruigiperda

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437996
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437996-500U
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      Description
      OverviewRecombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. 5-LO catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.
      Catalogue Number437996
      Brand Family Calbiochem®
      Synonyms5-LO
      References
      ReferencesBrock, T.G., et al. 1997. J. Biol. Chem. 272, 8276.
      Pouliot, M., et al. 1996. Eur. J. Biochem. 238, 250.
      Zhang, Y.Y., et al. 1993. J. Biol. Chem. 268, 2535.
      Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 689.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl₂, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.
      EC number1.13.11.34
      FormLiquid
      FormulationIn 100 mM Tris containing 5 mM EGTA, pH 8.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity~20 units/mg
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      5-Lipoxygenase, Human, Recombinant, S. fruigiperda SDS

      Title

      Safety Data Sheet (SDS) 

      5-Lipoxygenase, Human, Recombinant, S. fruigiperda Certificates of Analysis

      TitleLot Number
      437996

      References

      Reference overview
      Brock, T.G., et al. 1997. J. Biol. Chem. 272, 8276.
      Pouliot, M., et al. 1996. Eur. J. Biochem. 238, 250.
      Zhang, Y.Y., et al. 1993. J. Biol. Chem. 268, 2535.
      Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 689.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2008 RFH
      Synonyms5-LO
      DescriptionRecombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. It is localized in the cytosol of resting neutrophils, and is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.
      FormLiquid
      FormulationIn 100 mM Tris containing 5 mM EGTA, pH 8.0.
      EC number1.13.11.34
      Specific activity~20 units/mg
      Unit definitionOne unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl₂, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesBrock, T.G., et al. 1997. J. Biol. Chem. 272, 8276.
      Pouliot, M., et al. 1996. Eur. J. Biochem. 238, 250.
      Zhang, Y.Y., et al. 1993. J. Biol. Chem. 268, 2535.
      Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 689.