219332 β-Catenin/Tcf Inhibitor III, iCRT3 - Calbiochem

Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₂₃H₂₆N₂O₂S

Pricing & Availability

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219332-10MG
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      Glass bottle 10 mg
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      Description
      OverviewA cell-permeable oxazole compound that acts as a selective CRT (β-catenin responsive transcription) inhibitor (IC50 = 8.2 nM against Wnt3a-dependent SuperTopFlash STF16-luc reporter activity in HEK293 cultures) by interfering with β-cat-TCF4 interaction via direct β-catenin (β-cat) binding, while exhibiting much reduced activity against Hedgehog, Jak/Stat, or Notch signaling pathway-dependent transcription activation. Effectively inhibits cellular expression of CRT target genes, including WISP1, AXN2, and CYCD1, in a dose-dependent manner (25 to 75 µM), and selectively inhibits the proliferation of HCT116 (75 µM), HT29 (75 µM), and several primary human colon cancer cultures (6.25 to 100 µg/ml; average IC50 ~36 µg/ml) that require sustained Wnt/CRT activity for survival.
      Catalogue Number219332
      Brand Family Calbiochem®
      Synonyms2-(((2-(4-Ethylphenyl)-5-methyl-1,3-oxazol-4-yl)methyl)sulfanyl)-N-(2-phenylethyl)acetamide, Inhibitor of β-Catenin Responsive Transcription, Wnt Pathway Inhibitor VIII, C3
      References
      ReferencesLee, E., et al. 2013. Proc. Natl. Acad. Sci. USA 110, 15710.
      Gonsalves, F.C., et al. 2011. Proc. Natl. Acad. Sci. USA 108, 5954.
      Product Information
      FormTan powder
      Hill FormulaC₂₃H₂₆N₂O₂S
      Chemical formulaC₂₃H₂₆N₂O₂S
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      β-Catenin/Tcf Inhibitor III, iCRT3 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      β-Catenin/Tcf Inhibitor III, iCRT3 - Calbiochem Certificates of Analysis

      TitleLot Number
      219332

      References

      Reference overview
      Lee, E., et al. 2013. Proc. Natl. Acad. Sci. USA 110, 15710.
      Gonsalves, F.C., et al. 2011. Proc. Natl. Acad. Sci. USA 108, 5954.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-October-2013 JSW
      Synonyms2-(((2-(4-Ethylphenyl)-5-methyl-1,3-oxazol-4-yl)methyl)sulfanyl)-N-(2-phenylethyl)acetamide, Inhibitor of β-Catenin Responsive Transcription, Wnt Pathway Inhibitor VIII, C3
      DescriptionA cell-permeable oxazole compound that acts as a selective CRT (β-catenin responsive transcription) inhibitor (IC50 = 8.2 nM against Wnt3a-dependent SuperTopFlash STF16-luc reporter activity in HEK293 cultures) by interfering with β-cat-TCF4 interaction via direct β-catenin (β-cat) binding, while exhibiting much reduced activity against Hedgehog, Jak/Stat, or Notch signaling pathway-dependent transcription activation. Effectively inhibits cellular expression of CRT target genes, including WISP1, AXN2, and CYCD1, in a dose-dependent manner (25 to 75 µM), and selectively inhibits the proliferation of HCT116 (75 µM), HT29 (75 µM), and several primary human colon cancer cultures (6.25 to 100 µg/ml; average IC50 ~36 µg/ml) that require sustained Wnt/CRT activity for survival.
      FormTan powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₃H₂₆N₂O₂S
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (50 mg/ml)
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesLee, E., et al. 2013. Proc. Natl. Acad. Sci. USA 110, 15710.
      Gonsalves, F.C., et al. 2011. Proc. Natl. Acad. Sci. USA 108, 5954.