Single Molecule Counting (SMC™) Immunoassay Technology

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Introducing the SMCxPRO™ System
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Learn more about the SMCxPRO™ technology
and its uses in biomarker analysis.



In the News
Accelerating Drug Development & Clinical Validation with Single Molecule Counting

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Unleashing the Sensitivity of Single Molecule Counting (SMC™) Technology

Ultrasensitive SMC™ technology, originally developed by Singulex®, Inc., provides an indispensable tool in the researcher’s arsenal to help them move novel biology forward, fueling the discovery and development of new therapeutics.

MilliporeSigma now offers the power of SMC™ technology to leading pharmaceutical R&D laboratories, clinical research organizations (CROs) and academic institutions around the world through the Research-Use-Only SMCxPRO™ and Erenna® immunoassay systems, immunoassay kits, reagents, and custom services.

Singulex Erenna® Instrument
SMC™ Erenna® Instrument with SMC™ technology quantifies low-abundance biomarkers, on the bench top.

Previously Undetectable, Now Quantified. One Molecule at a Time.

SMC™ Single Molecule Counting (SMC™) technology enables precise measurement of molecules at levels previously undetectable, for unprecedented biomarker insights.

Scientists familiar with traditional immunoassays, such as ELISAs, know that sensitivity, matrix effects, and dynamic range affect how they make measurements—or if they can make them at all.

Combining a traditional immunoassay workflow with patented SMC™ technology enables the detection of low-abundance biomarkers, such as proteins and nucleic acids, with unparalleled sensitivity and accuracy, capturing concentrations down to the femtogram/mL level. Researchers can now detect, and monitor changes in, extremely low levels of established disease biomarkers such as cardiac troponin I and cytokines.

At femtograms per mL, it was undetectable…
Learn why Dr. Erika Cline switched to Singulex Erenna® assays.

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What Can Better Precision and Sensitivity Do for Your Research?

  • Quantify previously undetectable analytes
  • Better stratify sample populations
  • Gain insights into novel biological mechanisms
  • Require fewer data points for critical decision making
  • Accelerate drug discovery and development
  • Reduce program costs and improve productivity

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SMC™ Assay Workflow. During the capture and detection steps, specific antibodies translate each biomarker into signal. During the modified elution step, fluorescent dye-labeled detection antibodies are dissociated from the immunocomplex. The eluate is measured in a small interrogation space illuminated by a laser. Single fluorescently labeled molecules are excited by the laser as they pass through the interrogation space as detected events.

The SMCxPRO™ and Erenna® instruments capture the sum of all digital events counted above a background threshold. This model can have limited dynamic range so at higher concentrations on the Erenna® a proprietary algorithm computes the total sum of all photons recorded as an additional standard curve and the SMCxPRO™ digital event algorithm computes digital events across a spectrum of time series for a single standard curve thus improving assay sensitivity and extending the dynamic measuring range beyond what can be achieved with traditional technologies.