|G418 sulfate (G418)
G418 sulfate (G418) is an aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells, G418 is widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanrgenes.
Hygromycin B displays broad spectrum activity against prokaryotes and eukaryotes by strongly inhibiting protein synthesis.
An aminoglycoside, neomycin inhibits protein synthesis by binding to the ribosome, interfering with translation intitiation, inducing misreading, and promoting breakup of ribosomal polysomes.