Pharmaceutical Analysis: Corticoids


Column LiChrospher® 100 RP-18 (5 μm) LiChroCART® 125-4 Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-Chrom-CIA LiChrospher RP18 corticoids-480x436-02212014.png
Mobile phase Acetonitrile/0.5 mmol/l sodium acetate buffer 30/70 (v/v)
Flow rate 0.8 mL/min
Detection UV 235 nm
Temperature Room temperature
Injection volume 100 μl
Sample 1. Prednisolone
2. Cortisone
3. Dexamethasone
4. Prednisolone acetate
5. Hydrocortisone acetate
6. Cortisone acetate