Pharmaceutical Analysis: 2-Oxoacids


Column LiChrospher® 100 RP-18 (5 μm) LiChroCART® 250-4 Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-Chrom-CIA LiChrospher RP18 oxoacids-480x425-02212014.png
Mobile phase Methanol/Water/Acetonitrile 35/45/20 (v/v/v)
Flow rate 1.0 mL/min
Detection Fluorescence Ex 350 nm, Em 410 nm
Temperature Room temperature
Injection volume 100 μl
Sample 1. Pyruvate
2. -
3. 2-Oxobutyric acid
4. 2-Oxoisovaleric acid (from valine)
5. 2-Oxoisocaproic acid (from leucine)
6. 2-Oxocaproic acid (internal standard)