Pharmaceutical Analysis: Acetyl Salicylic Acid


Column LiChrospher® 100 RP-18 (5 μm) LiChroCART® 250-4 Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-Chrom-CIA LiChrospher RP18 acetyl-480x425-02212014.png
Mobile phase 0.01 mol/L sodium dihydrogen phosphate buffer, pH 2.0 with phosphoric acid/Acetonitrile/Methanol
70/25/5 (v/v/v)
Flow rate 1.0 mL/min
Detection UV 237nm
Temperature Room temperature
Injection volume 100 μl
Sample 1. Acetylsalicylic acid
2. Salicylic acid
3. p-Hydroxybenzoic acid