Chromolith® Column Coupler
Chromolith® HPLC Column Coupler |
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Perfect Combinations for Complex Mixtures
The Chromolith® HPLC column coupler is designed for combining several monolithic columns together in order to further increase separation efficiency and column performance. The combination can result in a theoretical plate count that is significantly higher than that of particulate columns. At the same time, pressure is kept well below the HPLC system limit.
The superior column performance achieved by using the Chromolith® HPLC column coupler allows you to solve highly critical separation problems in which resolution is a limiting factor. This makes column coupling perfect for chromatographic separations of hitherto non-separable, complex mixtures.
Typical Column Efficiency Using the Chromolith® HPLC Column Coupler
The table below shows a comparison between Chromolith® HPLC columns and particulate columns. The combination of just two Chromolith® Performance RP-18 endcapped columns yields a separation efficiency of 19,000 theoretical plates per column, which is usually the maximum for particulate columns.
Column |
Length [mm] |
Pressure [bar] |
Plate number per column [Anthracene] |
|---|---|---|---|
|
Chromolith® Performance 1x |
100 | 30 | 10,000 |
|
Chromolith® Performance 2x |
200 | 60 | 19,000 |
|
Chromolith® Performance 3x |
300 | 90 | 27,000 |
|
Chromolith® Performance 4x |
400 | 120 | 35,000 |
| Chromolith® Performance 5x | 500 | 150 | 41,000 |
| Particulate column (5 μm) |
250 | 220 | 18,500 |
| Particulate column (3.5 μm) | 150 | 400 | 19,000 |
Separation of 7 Alkenes Using Coupled Chromolith® Performance RP-18e Columns (81,000 Plates at 85 Bar Pressure)
| Column | Column 10 columns of Chromolith® Performance RP-18e, 100-4.6 mm | ||
|---|---|---|---|
| Mobile phase | 80 /20 Acetonitrile/water | ||
| Flow rate | 1 mL/min | ||
| Detection | UV 254 nm | ||
| Temperature | ambient | ||
| Injection volume | 10 μl | ||
| Sample |
1. Thiourea |
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Separation of 11 Steroids Using Coupled Chromolith® HighResolution RP-18e Columns
| Column | Chromolith® HighResolution RP-18e 2 x 100-4.6 mm / 1 x 50-4.6 mm | ||
|---|---|---|---|
| Mobile phase | ACN / water | ||
| Gradient | Time/min | %A | %B |
| 0 | 55 | 45 | |
| 2 | 95 | 5 | |
| 15 | 95 | 5 | |
| Flow rate | 1 mL/min | ||
| Pressure | 30 - 68 bar | ||
| Detection | UV 240 nm | ||
| LC System | LaChrom® L7000 | ||
| Vol. detector cell | 16 μL | ||
| Temperature | ambient | ||
| Injection volume | 10 μl | ||
| Sample |
1. Fluoxymesterone |
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