71576 pTriEx™-6 3C/LIC Vector Kit - Novagen

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      OverviewThe pTriEx™-6 3C/LIC vector is a 3C/LIC version of pTriEx-6. The pTriEx-6 3C/LIC vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect, and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, the pTriEx-6 3C/LIC vector contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector® System or BacMagic™ Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. The pTriEx-6 3C/LIC vector contains an N-terminal 8 aa Strep•Tag® II coding sequence followed by a HRV 3C protease cleavage site and a multiple cloning site, with the option to encode optional sequences for a C-terminal thrombin cleavage site and a His•Tag® (10-histidine) fusion.

      * Strep•Tag® technology for protein purification and detection is covered under US Patent No. 5,506,121, UK Patent No. 2,272,698 and French Patent No. 93 13 066. Strep•Tactin® is covered by US Patent No. 6,103,493. These products are provided only for research use. Information about licenses for commercial use is available from IBA GmbH, Rudolf-Wissell-Str. 28, D-37079 Goettingen, Germany.

      The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number71576
      Brand Family Novagen®
      Product Information
      1 µgpTriEx-6 3C/LIC Vector
      8 µl3C/LIC β-gal Control Insert
      25 UT4 DNA Polymerase (LIC-qualified)
      50 µl10X T4 DNA Polymerase Buffer
      100 µl100 mM DTT
      40 µl25 mM dATP
      50 µl25 mM EDTA
      1.5 mlNuclease-free Water
      22 × 50 µlNovaBlue GigaSingles™ Competent Cells
      2 × 0.2 mlOrigami™ B(DE3)pLacI Competent Cells
      5 × 2 mlSOC Medium
      10 µlTest Plasmid (ampicillin resistant)
      Quality LevelMQ100
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information

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