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71363 pRSF-1b DNA - Novagen

71363
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      Description
      OverviewThe pRSF-1b plasmid features coexpression capabilities as well as the ability to express fusion proteins with a N-terminal His•Tag® coding sequence that results in native protein after purification and cleavage. This plasmid carries an origin derived from RSF1030 (1, 2) and kanamycin resistance, allowing for the option of coexpression with many other Novagen T7-based expression vectors. Please read technical bulletin, TB401, for all possibilities and contact technical service if you need additional information.

      This plasmid contains a strong T7lac promoter, an amino-terminal His•Tag coding sequence, and multiple cloning site (MCS) regions designed to allow the generation of target proteins with minimal vector encoded fusion. The Pml I cloning site allows direct fusion to the His•Tag sequence for inserts that are blunt and in the appropriate reading frame. For applications requiring a removable amino-terminal His•Tag sequence, the MCS includes a PshA I cloning site (GACNNNNGTC). The PshA I site overlaps the cleavage site for the enterokinase (EK) protease (AspAspAspAspLys). Cloning appropriately designed inserts into this site re-creates the full EK site and allows all amino-terminal vector-encoded sequences to be removed by EK digestion. The remainder of the MCS encodes restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional S•Tag™ coding sequence is present at the distal end of the MCS for generating a carboxy-terminal tag compatible with purification, detection, and quantification (3).
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number71363
      Brand Family Novagen®
      References
      References1. Som, T. and Tomizawa, J. (1982) Mol. Gen. Genet. 187, 375–383.

      2. Cannon, P.M. and Strike, P. (1992) Plasmid 27, 220–230.

      3. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
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      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
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      Supplemental Information
      Specifications

      Documentation

      pRSF-1b DNA - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      pRSF-1b DNA - Novagen Certificates of Analysis

      TitleLot Number
      71363

      References

      Reference overview
      1. Som, T. and Tomizawa, J. (1982) Mol. Gen. Genet. 187, 375–383.

      2. Cannon, P.M. and Strike, P. (1992) Plasmid 27, 220–230.

      3. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.

      Citations

      Title
    • Jingjing Xie, et al. (2007) Hexameric calgranulin C (S100A12) binds to the receptor for advanced glycated end products (RAGE) using symmetric hydrophobic target-binding patches. Journal of Biological Chemistry 282, 4218-4231.
    • Olivier Berteau, et al. (2006) A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes. Journal of Biological Chemistry 281, 22464-22470.
    • User Protocols

      Title
      TB401 pRSF and pCDF Vectors

      Vector Map

      Title
      TB393VM pRSF-1b Vector Map

      Vector Sequence

      Title
      pRSF-1b Vector Sequence