71335 pET-46 Ek/LIC Vector Kit - Novagen

71335
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      Description
      OverviewThe pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source of T7 RNA polymerase in the host cell. T7 RNA polymerase is so selective and active that, when fully induced, almost all of the cell's resources are converted to target gene expression; the desired product can comprise more than 50% of the total cell protein a few hours after induction. All of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins.
      The pET-46 Ek/LIC Vector is a LIC-compatible derivative of pET-45b(+). The plasmid contains a strong T7lac promoter and an amino-terminal His•Tag® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet™ Adaptors for coexpression of two proteins as well as with pCDF-2 and pRSF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number71335
      Brand Family Novagen®
      References
      References1. Kim, J.S. and Raines, R.T. 1993. Protein Science 2, 348.
      Product Information
      1 µgEk/LIC Vector
      25 unitsT4 DNA Polymerase, LIC-qualified
      50 µl25 mM EDTA
      40 µl25 mM dATP
      8 µlEk/LIC β-gal Control Insert
      100 µl100 mM DTT
      50 µl10X T4 DNA Polymerase Buffer
      1.5 mlNuclease-free Water
      5 × 2 mlSOC Medium
      10 µlTest Plasmid
      0.2 mlBL21(DE3)pLysS Competent Cells
      0.2 mlBL21(DE3) Competent Cells
      22 × 50 µlNovaBlue GigaSingles™ Competent Cells
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pET-46 Ek/LIC Vector Kit - Novagen Certificates of Analysis

      TitleLot Number
      71335

      References

      Reference overview
      1. Kim, J.S. and Raines, R.T. 1993. Protein Science 2, 348.

      Citations

      Title
    • Park, M-O., et al. 2007. Journal of Bacteriology 189, 7281.
    • Smialowski, P., et al. 2007. Bioinformatics 23, 2536.
    • Yu, Z., et al. 2007. Nucleic Acids Research 35, 2107.
    • User Protocols

      Title
      TB055 pET System Manual
      TB163 Ek/LIC Cloning Kits
      TB184 Xa/LIC Cloning Kits

      Vector Map

      Title
      TB397VM pET-46 Ek/LIC Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors