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69077 pET-30 Ek/LIC Vector Kit - Novagen

69077
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      Description
      OverviewThe pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Ek protease cleavage site (AspAspAspAspLys↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69077
      Brand Family Novagen®
      Application Data
      References
      Product Information
      Components
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
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      Specifications

      Documentation

      pET-30 Ek/LIC Vector Kit - Novagen Certificates of Analysis

      TitleLot Number
      69077

      Citations

      Title
    • Rui He, et al. (2008) Metacaspase-8 modulates programmed cell death induced by ultraviolet light and H2O2 in Arabidopsis. Journal of Biological Chemistry 283, 774-783.
    • Steven G. Van Lanen, Shuangjun Lin and Ben Shen. (2008) Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism. Procedings of the National Academy of Science 105, 494-499.
    • Anette Thysse Jonstrup, et al. (2007) The 1.4-Å crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme. Nucleic Acids Research 35, 3153-3164.
    • Mads Gravers Jeppesen, et al. (2005) Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex. Journal of Biological Chemistry 280, 5071-5081.
    • Francisco J. Sandoval and Sanja Roje. (2005) An FMN hydrolase is fused to a riboflavin kinase homolog in plants. Journal of Biological Chemistry 280, 38337-38345.
    • Jongmin Lee, et al. (2004) Transglutaminase 2 induces nuclear factor-κB activation via a novel pathway in BV-2 microglia. Journal of Biological Chemistry 279, 53725-53735.
    • Beata Zalewska, et al. (2003) Chimeric Dr fimbriae with a herpes simplex virus type 1 epitope as a model for a recombinant vaccine. Infection and Immunity 71, 5505-5513.
    • User Protocols

      Title
      TB055 pET System Manual
      TB163 Ek/LIC Cloning Kits
      TB184 Xa/LIC Cloning Kits

      Vector Map

      Title
      TB162VM pET-30 Ek/LIC Vector Map

      Vector Sequence

      Title
      pET-30 Ek/LIC Vector Sequence

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors