69866 pET-28c(+) DNA - Novagen

69866
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pET-28a-c(+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag® configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB074). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69866
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Specifications

      Documentation

      pET-28c(+) DNA - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      pET-28c(+) DNA - Novagen Certificates of Analysis

      TitleLot Number
      69866

      Citations

      Title
    • Brian P. Anton, et al. (2008) RimO, a MiaB-like enzyme, methylthiolates the universally conserved Asp88 residue of ribosomal protein S12 in Escherichia coli. Procedings of the National Academy of Science 105, 1826-1831.
    • Maximilian J. Hartl, et al. (2008) AZT resistance of simian foamy virus reverse transcriptase is based on the excision of AZTMP in the presence of ATP. Nucleic Acids Research 36, 1009-1016.
    • Sean Crosson, et al. (2005) Conserved modular design of an oxygen sensory/signaling network with species-specific output. Proceedings of the National Academy of Sciences (USA) 102, 8018-8023.
    • Marie Futter, et al. (2005) Phosphorylation of spinophilin by ERK and cyclin-dependent PK 5 (Cdk5). Procedings of the National Academy of Science 102, 3489-3494.
    • Serban P. Georgescu, et al. (2005) Modulator recognition factor 1 (MRF1), an ARID-family member, is a novel co-repressor for estrogen receptor α. Molecular Endocrinology 19, 2491-2501.
    • Toshiyuki Mori, et al. (2005) Isolation and characterization of griffithsin, a novel HIV-inactivating protein from the red alga Griffithsia sp. Journal of Biological Chemistry 280, 9345-9353.
    • Shogo Kurebayashi, et al. (2004) Selective LXXLL peptides antagonize transcriptional activation by the retinoid-related orphan receptor RORc. Biochemical and Biophysical Research Communications 315, 919-927.
    • H. Zhong, et al. (2002) The heteromeric cyclic nucleotide-gated channel adopts a 3A:1B stoichemistry. 420, 193-198.
    • M.M. Penas, et al. (1998) Identification, characterization, and in situ detection of a fruit-body-specific hydrophobin of Pleurotus ostreatus. Applied and Enviornmental Microbiology 64, 4028-4034.
    • Feng Qian, et al. (1997) PKD1 interacts with PKD2 through a probable coiled-coil domain. Nature Genetics 16, 179-183.
    • H. Zhou, et al. (1997) CIITA-dependant and -independant class II MHC expression revealed by a dominant negative mutation. Journal of Immunology 158, 4741-4749.
    • John M. Hawdon, et al. (1996) Cloning and characterization of Ancylostoma-secreted protein: a novel protein associated with the transition to parasitism by infective hookworm larvae. Journal of Biological Chemistry 271, 6672-6678.
    • User Protocols

      Title
      TB055 pET System Manual

      Vector Map

      Title
      TB074VM pET-28a-c(+) Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors