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69862 pET-26b(+) DNA - Novagen

69862
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pET-26b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB071).

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69862
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pET-26b(+) DNA - Novagen Certificates of Analysis

      TitleLot Number
      69862

      Citations

      Title
    • Wu. L., et al. (2009) Structural Basis for Proteolytic Specificity of the Human Apoptosis-Inducing Granzyme M J. Immunol. 183, 421.
    • Damien Bessière, et al. (2008) Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways. Journal of Biological Chemistry 283, 4352-4363.
    • Nobutaka Funa, Takayoshi Awakawa and Sueharu Horinouchi. (2007) Pentaketide resorcylic acid synthesis by Type III polyketide synthase from Neurospora crassa. Journal of Biological Chemistry 282, 14476-14481.
    • Yongchao Li, Diana C. Irwin and David B. Wilson. (2007) Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A. Applied and Enviornmental Microbiology 73, 3165-3172.
    • Jean-Michel Heraud, et al. (2006) Subunit recombinant vaccine protects against monkeypox. Journal of immunology 177, 2552-2564.
    • Stan J. J. Brouns, et al. (2005) Engineering a selectable marker for hyperthermophiles. Journal of Biological Chemistry 280, 11422-11431.
    • Nobutaka Funa, et al. (2005) A novel quinone-forming monooxygenase family involved in modification of aromatic polyketides. Journal of Biological Chemistry 280, 14514-14523.
    • Ryohei Ishii, et al. (2005) Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima. Journal of Biological Chemistry 280, 14138-14144.
    • Hongmin Li, et al. (2005) Crystal structures of T cell receptor β chains related to rheumatoid arthritis. Protein Science 14, 3025-3038.
    • Taishi Tsubouchi, et al. (2005) Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. Journal of Biological Chemistry 280, 18511-18516.
    • Florence Vincent, Gideon J. Davies and James A. Brannigan. (2005) Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily? Journal of Biological Chemistry 280, 19649-19655.
    • Yu-hang Chen, et al. (2004) Structural basis of the α1-β subunit interaction of voltage-gated Ca2+ channels. 429, 675-680.
    • Watchrra Teartasin, et al. (2004) Expression, purification, and preliminary X-ray diffraction analysis of a ketoreductase from a type II polyketide synthase. Acta Crystallographica D60, 1137-1138.
    • Shalom D. Goldberg, et al. (2003) Identification of residues critical for catalysis in a class C b-lactamase by combinatorial scanning mutagenesis. Protein Science 12, 1633-1645.
    • User Protocols

      Title
      TB055 pET System Manual

      Vector Map

      Title
      TB071VM pET-26b(+) Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors