2910 Whole Cell Extraction Kit

2910
100 assays  
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      Overview

      Replacement Information
      Description
      Catalogue Number2910
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionWhole Cell Extraction Kit
      Materials Required but Not Delivered1. Cultured Cells and Tissue Culture Supplies

      2. Cell Detachment Buffer (Trypsin) or Cell Scrapers

      3. Phosphatase Inhibitors (if desired)

      4. Syringes, 1mL - 27 Gauge Needle

      5. 1.5 mL Microcentrifuge tubes

      6. Deionized Water

      7. Table-top Microcentrifuge, 4oC

      8. Phosphate-Buffered Saline (PBS), Tris-Buffered Saline (TBS), or HBSS (Hank's Balanced Salt Solution)
      References
      Product Information
      Components
      • Whole Cell Extraction Buffer, 5x: - (Part No. 90493) Two vials containing 10 mL of 5x concentrated buffer. Dilute to 1x final concentration with ddH2O prior to use.
      • Protease Inhibitor Cocktail: - (Part No. 90492) One vial containing 100μL of protease inhibitors in DMSO for use with mammalian cells and tissue extracts. A mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, and aspartic acid proteases and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, bestatin, leupeptin, and aprotinin. Contains no metal chelators. Prior to use, dilute 1/1000 in 1x Whole Cell Extraction Buffer.
      Applications
      Key Applications
      • Protein Extraction
      Application NotesCHEMICON®'s Whole Cell Extraction Kit (Catalog No. 2910) provides a simple and convenient method for the preparation of whole cell extractions for use in experimental studies of various cellular proteins.

      This kit is recommended for use with CHEMICON®'s Rac Activation Assay Kit (Catalog No. SGT425), Cdc42 Activation Assay Kit (Catalog No. SGT430), Ras Activation Assay Kit (Catalog No. SGT435), and CHEMICON®'s Transcription Factor Assays when purified nuclear extracts are not required.

      For Research Use Only; Not for use in diagnostic procedures
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions· The 5x Whole Cell Extraction Buffer can be stored at 2-8°C until expiration date.

      · The prepared 1x Whole Cell Extraction Buffer (with optional additives) should be stored at -20°C for up to one month.

      · The undiluted Protease Inhibitor Cocktail can be stored at -20°C until the expiration date.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications