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70625 Tuner™(DE3)pLacI Competent Cells - Novagen

70625
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70625-3
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      70625-4
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          Description
          OverviewExpression host strains for pETBlue™ and pTriEx™ vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.

          Note that these strains are not recommended for use with pET, pACYCDuet™, pCDF, pRSF, pCOLADuet™ or pETcoco™ vectors.

          .Tuner™ strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins. In Tuner(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.

          DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
          Genotype: F ompT hsdSB (rB mB) gal dcm lacY1(DE3) pLacI (CamR)


          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number70625
          Brand Family Novagen®
          References
          Product Information
          Components
          Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁶ cfu/µg
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Tuner™(DE3)pLacI Competent Cells - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          Tuner™(DE3)pLacI Competent Cells - Novagen Certificates of Analysis

          TitleLot Number
          70625

          Citations

          Title
        • Yefim Manevich, et al. (2007) Structure and phospholipase function of peroxiredoxin 6: Identification of the catalytic triad and its role in phospholipid substrate binding. Journal of Lipid Research 48, 2306-2318.
        • Tsui-Fen Chou, et al. (2005) 31P NMR and genetic analysis establish hinT as the only E. coli purine nucleoside phosphoramidase and as essential for growth under high salt conditions. Journal of Biological Chemistry 15356-15361.
        • Peter R. Nielsen, et al. (2005) Structure of the chromo barrel domain from the MOF acetyltransferase. Journal of Biological Chemistry 280, 32326-32331.
        • Michelle B. Ryndak, et al. (2005) Role of predicted transmembrane domains for type III translocation, pore formation, and signaling by theYersinia pseudotuberculosis YopB protein. Infection and Immunity 73, 2433-2443.
        • Michael D. Urbaniak, et al. (2005) The N-Acetyl-D-glucosaminylphosphatidylinositol De-N-acetylase of glycosylphosphatidylinositol biosynthesis Is a zinc metalloenzyme. Journal of Biological Chemistry 280, 22831-22838.
        • Cynthia L. Richard, Animesh Tandon and Robert G. Kranz. (2004) Rhodobacter capsulatus nifA1 promoter: high-GC -10 regions in High-GC bacteria and the basis for their transcription. Journal of Bacteriology 186, 740-749.
        • Marina V. Backer, et al. (2003) Engineering S-protein fragments of bovine ribonuclease A for targeted drug delivery. Protein Expression and Purification 26, 455-461.
        • Deanne M. Compaan and W. Ross Ellington. (2003) Functional consequences of a gene duplication and fusion event in an arginine kinase. 206, 1545-1556.
        • Michael Peitz, et al. (2002) Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: a tool for efficient genetic engineering of mammalian genomes. Procedings of the National Academy of Science 99, 4489-4494.
        • Sheng Yuan, Yajun Wu and Daniel J. Cosgrove. (2001) A fungal endoglucanase with plant cell wallextension activity. Plant Physiology 127, 324-333.
        • User Protocols

          Title
          TB249 pETBlue™ System Manual
          TB250 pTriEx™ System Manual