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111732 Trypan blue (C.I. 23850)

111732
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      Glass bottle 25 g
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      Description
      Catalogue Number111732
      SynonymsBenzamine blue, Tolidine-disazo-bis-8-amino-1-naphthol-3,6-disulfonic acid tetrasodium salt, 3,3'-Dimethyl-4,4'-bis(5-amino-4-hydroxy-2,7-disulfonaphthyl-3-azo)-[1,1'-biphenyl] tetrasodium salt
      DescriptionTRYPAN BLUE (C.I. 23850) FOR MICROSCOPY
      OverviewTrypan blue (C.I. 23850) is a dry dye for the preparation of a color staining solution, staining histological sample material of human origin, e.g. trichrome staining in collagenous connective tissue.
      The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.
      References
      Product Information
      CAS number72-57-1
      EC index number611-030-00-4
      EC number200-786-7
      Hill FormulaC₃₄H₂₄N₆Na₄O₁₄S₄
      Molar Mass960.79 g/mol
      HS Code3204 14 00
      Structure formula ImageStructure formula Image
      Quality LevelMQ300
      Applications
      ApplicationUsed in trichrome staining and to assess cell viability
      Biological Information
      Physicochemical Information
      Melting Point>300 °C (decomposition)
      pH value9.8 (10 g/l, H₂O, 20 °C)
      Bulk density800 kg/m3
      Solubility10 g/l
      Dimensions
      Materials Information
      Toxicological Information
      LD 50 oralLD50 Rat 6200 mg/kg
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Statement(s)H350: May cause cancer.
      Precautionary Statement(s)P201: Obtain special instructions before use.
      P308 + P313: IF exposed or concerned: Get medical advice/ attention.
      Signal WordDanger
      RTECSQJ6475000
      Storage class6.1C Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
      WGKWGK 3 highly hazardous to water
      Disposal9
      Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
      Safety Information
      Hazard Symbols Hazard SymbolsToxic
      Categories of dangercarcinogenic
      R PhraseR 45
      May cause cancer.
      S PhraseS 53-45
      Avoid exposure - obtain special instructions before use.In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +2°C to +30°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDKein Gefahrgut
      Declaration (transport by air) IATA-DGRNo Dangerous Good
      Declaration (transport by sea) IMDG-CodeNo Dangerous Good
      Supplemental Information
      Specifications
      Dye content (spectrophotometrically)≥ 80 %
      Identity
      - sodium

      passes test
      - UV/VIS-Spectrumpasses test
      Absorption maximum λ max (water)590 - 600 nm
      Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; water)650 - 820
      TLC-Testpasses test
      Loss on drying (110 °C)≤ 15 %
      Suitability for microscopypasses test