PF060 TdT, Calf Thymus

PF060
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PF060-.155ML
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      Plastic ampoule .155 ml
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      Description
      OverviewNative TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxy terminus of either double of single stranded DNA in a template-independent manner.
      Catalogue NumberPF060
      Brand Family Calbiochem®
      SynonymsTerminal Deoxynucleotidyl Transferase
      References
      ReferencesChang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
      Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
      Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
      Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
      Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
      Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
      Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.
      Product Information
      Unit of DefinitionOne unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3ʹ-OH terminus of d(A)50 in 60 min at 37°C.
      FormLiquid
      Formulation775 Units in 50 mM potassium phosphate buffer, 1 mM B-mercaptoethanol, 50% glycerol, pH 7.2.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications DNA Labeling
      Application NotesDNA Labeling
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      TdT, Calf Thymus SDS

      Title

      Safety Data Sheet (SDS) 

      TdT, Calf Thymus Certificates of Analysis

      TitleLot Number
      PF060

      References

      Reference overview
      Chang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
      Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
      Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
      Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
      Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
      Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
      Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsTerminal Deoxynucleotidyl Transferase
      ApplicationDNA Labeling
      DescriptionNative TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxy terminus of either double of single stranded DNA in a template-independent manner. Although TdT preferentially adds deoxynucleotides to 3'-extensions, tailing onto 5' overhangs or blunt ended double strands of DNA can also be achieved. TdT can be used for labeling DNA fragments and vectors with homopolymer tails and/or with modified deoxynucleotides such as biotin-dNTPs, 32P-dNTPs, cordycepin-dNTPs, or ddNTP.
      BackgroundThe Calbiochem® Terminal deoxynucleotidyl Transferase (TdT) is a DNA polymerase isolated from calf thymus. TdT catalyzes the addition of deoxynucleotides to the 3'-hydroxyl termini of either double or single stranded DNA molecules in a template independent manner. Although TdT preferentially adds deoxynucleotides to 3'-extensions, tailing onto 5' overhangs or blunt ended double strands of DNA can also be achieved. TdT can be used for labeling DNA fragments and vectors with homopolymer tails and/or with modified deoxynucleotides such as biotin-dNTPs, 32P-dNTPs, cordycepin-dNTPs, or ddNTP.
      FormLiquid
      Formulation775 Units in 50 mM potassium phosphate buffer, 1 mM B-mercaptoethanol, 50% glycerol, pH 7.2.
      Unit definitionOne unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3ʹ-OH terminus of d(A)50 in 60 min at 37°C.
      PreservativeNone
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesChang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
      Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
      Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
      Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
      Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
      Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
      Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.