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616450 TGF-β1, Human Platelets

616450
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Overview

Replacement Information

Key Specifications Table

Purity
≥97% by SDS-PAGE

Pricing & Availability

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616450-1UG
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      Glass bottle 1 μg
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      Description
      OverviewNative transforming growth factor-β1 from human platelets. Promotes apoptosis in resting human B lymphocytes, glioma cells, and trigeminal neurinomal cells.
      Catalogue Number616450
      Brand Family Calbiochem®
      SynonymshTGF-β1, Transforming Growth Factor-β1
      References
      ReferencesLomo, J., et al. 1995. J. Immunol. 154, 1634.
      Marushige, K. and Marushige, Y. 1995. Anticancer Res. 14, 2419.
      Bermundez, L.E. 1993. J. Immunol. 150, 1838.
      Hamilton, J.A., et al. 1993. J. Immunol. 150, 1496.
      Reilly, J.T., et al. 1993. Br. J. Haematol. 83, 58.
      Sporn, M.B. and Roberts, A.B. 1993. Growth Factors 8, 1.
      Product Information
      FormLyophilized
      FormulationLyophilized from a sterile-filtered solution of 50 µg BSA/µg TGF-β1, 35% acetonitrile, 0.1% TFA.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 40-10 pg/ml as measured by the inhibition of IL-4-dependant proliferation of mouse HT-2 cells using resazurin dye as an indicator of cell proliferation.
      Purity≥97% by SDS-PAGE
      SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 EU/ug of TGF-β1
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C) for short-term storage or freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 months at 4°C or up to 3 months at -20°C..
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      TGF-β1, Human Platelets SDS

      Title

      Safety Data Sheet (SDS) 

      TGF-β1, Human Platelets Certificates of Analysis

      TitleLot Number
      616450

      References

      Reference overview
      Lomo, J., et al. 1995. J. Immunol. 154, 1634.
      Marushige, K. and Marushige, Y. 1995. Anticancer Res. 14, 2419.
      Bermundez, L.E. 1993. J. Immunol. 150, 1838.
      Hamilton, J.A., et al. 1993. J. Immunol. 150, 1496.
      Reilly, J.T., et al. 1993. Br. J. Haematol. 83, 58.
      Sporn, M.B. and Roberts, A.B. 1993. Growth Factors 8, 1.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-May-2008 RFH
      SynonymshTGF-β1, Transforming Growth Factor-β1
      DescriptionNative transforming growth factor-β1 from human platelets. Promotes apoptosis in resting human B lymphocytes, glioma cells, and trigerminal neurinomal cells.
      FormLyophilized
      FormulationLyophilized from a sterile-filtered solution of 50 µg BSA/µg TGF-β1, 35% acetonitrile, 0.1% TFA.
      SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1 EU/ug of TGF-β1
      Biological activityED₅₀ = 40-10 pg/ml as measured by the inhibition of IL-4-dependant proliferation of mouse HT-2 cells using resazurin dye as an indicator of cell proliferation.
      SolubilityReconstitute to a concentration ≥10 µg/ml in sterile 4 mM HCl containing ≥0.1% HSA or BSA.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C) for short-term storage or freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 months at 4°C or up to 3 months at -20°C..
      Toxicity Standard Handling
      Merck USA index14, 9572
      ReferencesLomo, J., et al. 1995. J. Immunol. 154, 1634.
      Marushige, K. and Marushige, Y. 1995. Anticancer Res. 14, 2419.
      Bermundez, L.E. 1993. J. Immunol. 150, 1838.
      Hamilton, J.A., et al. 1993. J. Immunol. 150, 1496.
      Reilly, J.T., et al. 1993. Br. J. Haematol. 83, 58.
      Sporn, M.B. and Roberts, A.B. 1993. Growth Factors 8, 1.

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors