70014 T7Select® Packaging Kit

70014
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      Description
      OverviewNovagen's T7Select® Phage Display System is a novel phage display system that takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to display peptides up to about 50 amino acids in size in high copy number (415 per phage), and peptides or proteins up to about 1200 amino acids in low copy number (0.1-1 per phage) or mid-copy number (5-15 per phage). Phage assembly takes place in the E. coli cytoplasm and mature phage are released by cell lysis. Unlike the filamentous systems, peptides or proteins displayed on the surface of T7 do not need to be capable of secretion through the cell membrane, a necessary step in filamentous phage assembly.

      T7Select Packaging Extracts are optimized for maximum packaging efficiencies of T7Select vectors ligated with insert. The high efficiency extracts (> 2 x 108 pfu/µg with T7Select Packaging Control DNA) make it easy to produce a library containing at least 5 x 107 recombinants per microgram of arms. The extracts are made from a specially engineered phage that reduces the nonrecombinant cloning background to below 0.1%.

      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number70014
      Brand Family Novagen®
      References
      Product Information
      6T7 Packaging Extracts
      1 µgT7Select Packaging Control DNA
      0.2 mlBL21 glycerol stock
      0.2 mlBLT5403 glycerol stock
      0.2 mlBLT5615 glycerol stock
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      T7Select® Packaging Kit Certificates of Analysis

      TitleLot Number
      70014

      Citations

      Title
    • Rotem Edgar, et al. (2006) High-sensitivity bacterial detection using biotin-tagged phage and quantum-dot nanocomplexes. Proceedings of the National Academy of Sciences (USA) 103, 4841-4845.
    • Lauren R.H. Krumpe, et al. (2006) T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries. Proteomics 6, 4210-4222.
    • Felicity K. Kerr, et al. (2005) Elucidation of the substrate specificity of the C1s protease of the classical complement pathway. Journal of Biological Chemistry 280, 39510-39514.
    • Lianglin Zhang, Jason A. Hoffman and Erkki Ruoslahti. (2005) Molecular profiling of heart endothelial cells. Circulation Research 112, 1601-1611.
    • Zhaozhong Han, et al. (2002) Discovery of a stable dimeric mutant of cyanovirin-N (CV-N) from a T7 phage-displayed CV-N mutant library. Biochemical and Biophysical Research Communications 292, 1036-1043.
    • Kimmo Porkka, et al. (2002) A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo. Procedings of the National Academy of Science 99, 7444-7449.
    • M.P. Burgoon, et al. (2001) Antigen discovery in chronic human inflammatory central nervous disease: panning phage-display antigen libraries identifies the targets of central nervous system-derived IgG in subacute sclerosing panencephalitis. Journal of Immunology 167, 6009-6014.
    • Stefan Danner and Joel G. Belasco. (2001) T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries. Procedings of the National Academy of Science 98, 12954-12959.
    • Valerie A Fadok, et al. (2000) A receptor for phosphatidylserine-specific clearance of apoptotic cells. 405, 85-90.
    • Huiming Guo, et al. (2000) EMMPRIN (CD147), an inducer of matrix metalloproteinase synthesis, also binds interstitial collagenase to the tumor cell surface. Cancer Research 60, 888-891.
    • Hamid Houshmand, Gunnar Froman and Goran Magnusson. (1999) Use of bacteriophage T7 displayed peptides for determination of monoclonal antibody specificity and biosensor analysis. Analytical Biochemistry 268, 363-370.
    • Sara Nakielny, et al. (1999) Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain. European Molecular Biology Organization Journal 18, 1982-1995.
    • M. Yamamoto, Y. Kominato and F. Yamamoto. (1999) Phage display cDNA cloning of protein with carbohydrate affinity. Biochemical and Biophysical Research Communications 255, 194-199.
    • User Protocols

      Title
      TB178 T7Select® System Manual

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      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors
      Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Kits