|Stericap PLUS Universal Bottle-Top Filter Device|
|How can I clean the Stericap PLUS tubeset between uses? Is it autoclaveable?||The tubeset provided with the Stericap PLUS can be cleaned with detergent and water. Be certain to thoroughly rinse the tubeset if detergent is used. The tubing set which is provided is autoclave compatible for at least 10 cycles at 121oC for 15 minutes. After the first autoclave cycle, the tubing may lose some of its transparency and the tube weight will appear lighter in color, but this is purely cosmetic and does not affect its function nor lead to additional extractables.|
|What flow rates can I expect from my Stericap PLUS?||Stericap PLUS has a QC release specified clean water flow time of <52 seconds for 500 mL (minimum average flow rate of 577 mL/min) at a vacuum level of 25” Hg. Filtration of more viscous or particulate laden solutions will result in lower flow rates, and instantaneous flow rates that reduce over time. For example in competitive performance testing, average flow rate over 10 minutes for DMEM with 10% serum was 550 mL/min, while over 20 minutes it was 375 mL/minute, and over 60 minutes, it was 167 mL/min.|
|Can I bubble point my Stericap PLUS unit?||Since it is a vacuum driven device, Stericap PLUS is not configured to allow bubble point testing. The housing leak integrity test on the housing is performed at 15 psig, which is significantly lower than the water bubble point of the membrane.|
|Can I reuse my Stericap PLUS?||Stericap PLUS is a disposable filtration unit intended for single use only.|
|Which receiver bottles can I use with my Stericap PLUS?||Stericap PLUS is a universal bottle top filtration device. It is compatible with bottles which have neck inner diameter sizes as small as 20mm up to wide mouth bottles with inner neck diameter of 67mm. Any glass or plastic receiver bottle used should be rated for use with vacuum. Millipore offers disposable, plastic, vacuum rated 1 Liter receiver bottles (SC00B10RE).|
|For some unknown reason my Stericap PLUS suddenly stopped filtering. What could be the problem?||If you have a proper seal and adequate vacuum, you may have encountered an air lock of the unit. This event is caused by excess air entering the domed housing of the Stericap PLUS device. Follow these steps to recover from an airlock event which can also be found in your Stericap Plus User Guide:
1. Leave the vacuum source on and connected to the device in order to protect the membrane.
2. Attach a 50 cc syringe to the vent port needle valve’s female Luer-Lok™ connector on the top center of the dome.
3. Twist the needle valve counterclockwise slightly (1 to 1 1/2 turns) to open.
4. Pull on the syringe plunger to remove the air from the housing and re-prime the feed tube and housing with as much liquid as possible so that the membrane surface is fully submerged again.
NOTE: A small pocket of air at the top of the dome is normal and will not affect filtration.
5. Filtration should resume immediately. Return to step 4 of the “How to Use the Stericap PLUS Filter Unit” section.
|How long can I be certain that the Stericap PLUS is sterile after I purchase it?||Millipore guarantees sterility of the Stericap PLUS for 3 years from date of manufacture.|
|What is the hold up volume in the Stericap PLUS unit?||Stericap PLUS holdup volume is <20 mL.|
|Some liquid I was filtering was aspirated into my vacuum line. How do I prevent that from happening?||You should stop filtering before the liquid level reaches the top of the bottle to prevent liquid from entering the vacuum line. To protect you vacuum source from serious damage which can be caused when liquid accidentally enters the vacuum tubing, your setup should consist of both a trap followed by a Millex FG 50 (SLFG05010) hydrophobic vent filter.|
|I have to filter volumes larger than 10 liters. Does Millipore have other, similar products for larger volume filtration?||Millipore’s Sterivac unit is a universal bottle top vacuum filtration unit for volumes of 10-20 L, and Steripak pressure driven units for volumes of 10-20 L.|