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F8205-BC Sebomed Basal Medium

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      500 ml
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      OverviewModified DMEM/Ham's F-12 (1:1), was optimized to grow the human sebaceous gland cell line SZ95 in vitro. The cell line itself was developed by transfecting primary human facial sebocytes. This defined medium is ideal for controlled studies regarding the effects of hormones, cytokines,xenobiotics, Ca2+ etc. on proliferation, differentiation, lipid synthesis, and cytokine production in the cell culture system. The Ca2+ concentration was kept low (0.05 mM)in order to cultivate sebocytes, and keratinocytes under similar culture conditions with or without addition of Ca2+. The basal medium, which is free of bovine products, does not contain phenol red or linoleic acid (or any other fatty acid).


      Sterility: Pass
      BHK 21 Cell Proliferation Test: Pass
      BHK 21 Cell Cloning Efficicency Test: Pass
      pH: 7.1-7.4
      Osmolality: 0.275-0.305 Osm/kg H20
      Color: Light pink
      Appearance: Clear

      For optimal performance, this basal medium needs to be supplemented with 0.1 ng/ml Epidermal Growth Factor (EGF ; cat. no. W 1325950500), and 0.1 ml/ml Fetal Bovine Serum (FBS). Gentamycin sulphate (cat. no. A2710/2) at a concentration of 50 mg/ml should be used to protect against bacterial contamination or 100 U/100 mg/l Penicillin/Streptomycin (cat. no. A2210/2)
      Catalogue NumberF8205-BC
      Brand Family Calbiochem®
      Product Information
      Quality LevelMQ300
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      F8205 04027269840624