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69232 S•Tag™ Thrombin Purification Kit

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      OverviewThe S•Tag™ Thrombin Purification Kit is designed for rapid affinity purification of S•Tag fusion proteins that also contain a sequence for thrombin cleavage. The S-protein Agarose specifically retains S•Tag fusion proteins. When fusion proteins are expressed from vectors that also encode a thrombin cleavage site (LVPR/GS) between the standard 15-amino acid S•Tag sequence (KETAAAKFERQHMDS) and the cloning region, thrombin digestion can release the target protein from the S-protein Agarose. This kit uses biotinylated thrombin to enable simple and specific thrombin removal with Streptavidin-Agarose, after the digestion step.
      Catalogue Number69232
      Brand Family Novagen®
      Product Information
      2 mlS-protein Agarose
      3 × 5 ml10X Bind/Wash Buffer
      3 × 1 ml10X Thrombin Cleavage Buffer
      50 UBiotinylated Thrombin
      0.4 ml Streptavidin Agarose
      2 ea/pkgSpin Filters, 5 ml capacity
      Biological Information
      Physicochemical Information
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      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Yes
      Packaging Information
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      Supplemental Information


      S•Tag™ Thrombin Purification Kit Certificates of Analysis

      TitleLot Number


    • Christoph Edner, et al. (2007) Glucan, water dikinase activity stimulates breakdown of starch granules by plastidial beta-amylases. Plant Physiology 145, 17-28.
    • Manu Agarwal, et al. (2006) A R2R3-type myb transcription factor is involved in the cold-regulation of CBF genes and in acquired freezing tolerance. Journal of Biological Chemistry 281, 37636-37645.
    • Kunhong Xiao, et al. (2004) Activation-dependent conformational changes in β-arrestin 2. Journal of Biological Chemistry 279, 55744-55753.
    • User Protocols


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      Life Science Research > Protein Sample Preparation > Protein Purification > Kits & Enzymes for Fusion Tags