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03-0184-00 SMC™ Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit

03-0184-00
96 well assay  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Single Molecule Counting (SMC™)
      Description
      Catalogue Number03-0184-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit
      OverviewThe SMC™ Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationThe human tau family of microtubule-associated neuronal proteins has been rigorously studied in the context of normal and aberrant neurobiology. While typically involved in the stability of axonal microtubules, tau can also play a role in neurodegenerative diseases such as Alzheimer’s Disease (AD), where hyperphosphorylation of the protein and the resulting misfolding and aggregation can negatively impact neural communication. Today, the threonine-181 (T181) residue of tau is a key phosphorylation site that is examined for its role as an AD biomarker due to it being present in 95% of AD cases.
      References
      Product Information
      Components
      • Assay Buffer
      • pTau T181 Coated Beads
      • Standard Diluent
      • pTau T181 Detection Antibody
      • pTau T181 Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • The assay variations of SMC™ Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit were studied using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
      • Intra-assay CV% average : ≤ 7%
      • Inter-assay CV% average : ≤ 12%
      Applications
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Phospho-Tau (T181) in human serum, plasma, and CSF.
      Key Applications
      • Single Molecule Counting (SMC™)
      Application NotesThis new streamline kit has the following features including:
      • Improved options for sample prep and incubation
      • Lyophilization of the standard provides the kit at one storage condition
      • Increased reagent volumes to accommodate automation
      • Validated to run on both SMCxPRO™ and Erenna®
      Biological Information
      Species Reactivity NoteSpecificity was not tested.
      Cross ReactivityCross-reactivity was not tested.
      Accuracy
      • Minimum of 5 serum samples spiked with average recovery of 97% with a range of 70-138%
      • Minimum of 5 plasma samples spiked with average recovery of 97% with a range of 81-127%
      • Minimum of 5 CSF samples spiked with average recovery of 108% with a range of 82-146%
      Physicochemical Information
      Sensitivity
      • Lower Limit of Quantification: 0.94 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications