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03-0188-00 SMC™ Human Phospho-α-Synuclein (S129) High Sensitivity Immunoassay Kit

03-0188-00
96 well assay  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Single Molecule Counting (SMC™)
      Description
      Catalogue Number03-0188-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human Phospho-α-Synuclein (S129) High Sensitivity Immunoassay Kit
      OverviewThe SMC™ Human Phospho-α-Synuclein (S129) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationHuman α-synuclein, encoded by the gene SNCA, is a presynaptic neuronal protein that has been studied for its potential pathological link to neurodegenerative diseases, most notably Parkinson’s disease (PD), where this protein has been observed to undergo aberrant misfolding and aggregation within neurons. In recent years, there has been increasing evidence that phosphorylation of Serine-129 can be specifically observed in neuronal α-synuclein aggregations within PD patients, in a manner not seen in healthy brains. The SMC™ Human phospho-α-synuclein (S129) High Sensitivity Immunoassay Kit enables researchers to further their understanding of this protein as a potential neurodegenerative marker, by accurately measuring this analyte in human cerebrospinal fluid (CSF) and blood-based samples.
      References
      Product Information
      Components
      • Assay Buffer
      • Phospho-α-synuclein (S129) Coated Beads
      • Standard Diluent
      • Phospho-α-synuclein (S129) Detection Antibody
      • Phospho-α-synuclein (S129) Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • The assay variations of SMC™ Human Phospho-α-Synuclein (S129) Immunoassay kit were studied using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
      • Intra-assay CV% average : 6%
      • Inter-assay CV% average : 7%
      Applications
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human phospho-α-synuclein (S129) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of phospho-α-synuclein (S129) in human CSF, serum, and plasma.
      Key Applications
      • Single Molecule Counting (SMC™)
      Application NotesThis new streamline kit has the following features including:
      • Improved options for sample prep and incubation
      • Lyophilization of the standard provides the kit at one storage condition
      • Increased reagent volumes to accommodate automation
      • Validated to run on both SMCxPRO™ and Erenna®
      Biological Information
      Species Reactivity NoteSpecificity to the following species analytes were tested with the following results:
      Monkey – 100%
      Cat – 100%
      Rat – 100%
      Mouse – 25%
      Beagle – 25%
      Cross ReactivityNo cross-reactivity was observed to an alpha-synuclein recombinant protein purified from E. Coli (i.e. non-phosphorylated alpha-synuclein).
      Accuracy
      • CSF Recovery: 5 CSF samples spiked with average recovery of 100% and a range of 95-103%
      • Serum/Plasma Recovery: 2 serum samples and 3 plasma samples with average recovery of 87% and a range of 83-91%
      Physicochemical Information
      Sensitivity
      • Lower Limit of Quantification: 0.1 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications