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03-0191-00 SMC™ Human IFN-β1 High Sensitivity Immunoassay Kit

96 well assay  
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      Key Specifications Table

      Key Applications
      Single Molecule Counting (SMC™)
      Catalogue Number03-0191-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human IFN-β1 High Sensitivity Immunoassay Kit
      OverviewThe SMC™ Human IFN-β1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationA member of the type I interferon family of glycoprotein second messengers, human Interferon Beta (IFN-β1) has been associated with antiviral activity as a secreted factor from infected cells that mediates innate immunity in neighboring cells. In addition, IFN-β1 has also been utilized as treatment for Multiple Sclerosis (MS) due to its ability to ameliorate inflammation in the central nervous system in this disease.
      Product Information
      • Assay Buffer
      • IFN-β1 Coated Beads
      • Standard Diluent
      • IFN-β1 Detection Antibody
      • IFN-β1 Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • The assay variations of SMC™ Human IFN-β1 Immunoassay kit were studied using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
      • Intra-assay CV% average : 6%
      • Inter-assay CV% average : 13%
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IFN-β1 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IFN-β1 in human serum and plasma.
      Key Applications
      • Single Molecule Counting (SMC™)
      Application NotesThis new streamline kit has the following features including:
      • Improved options for sample prep and incubation
      • Lyophilization of the standard provides the kit at one storage condition
      • Increased reagent volumes to accommodate automation
      • Validated to run on both SMCxPRO™ and Erenna®
      Biological Information
      Species Reactivity NoteSpecificity to the following species recombinant proteins were tested with the following results:
      Feline – 0.5% Mouse – 4% Rat – 0.3%
      Cross ReactivityCross-reactivity to the following analytes were tested with the following results:
       human IFN-α2 – 0.2%
       human IFN-γ – 0.3%
       human IL-29 (IFN-λ1) – 0.2%
       human IL-28A (IFN-λ2) – 0.2%
       human IL-28B (IFN-λ3) – 0.3%
       human IFN-ω – 0.2%
      • Minimum of 5 serum samples spiked with average recovery of 88% with a range of 78-100%
      • Minimum of 5 plasma samples spiked with average recovery of 93% with a range of 75-113%
      Physicochemical Information
      • Lower Limit of Quantification: 0.15 pg/mL
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information