Millipore Sigma Vibrant Logo

SCM005 ReNcell® NSC Maintenance Media

SCM005
500 mL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCM005
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionReNcell® NSC Maintenance Media
      OverviewReNcell® NSC Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell® immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell® immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell® NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell® immortalized neural progenitor cells
      Materials Required but Not Delivered1. ReNcell® CX Immortalized Cell Line (CHEMICON Cat. No. SCC007)
      2. ReNcell® VM Immortalized Cell Line (CHEMICON Cat. No. SCC008)
      3. Basic fibroblast growth factor (bFGF; FGF-2; Specific Activity greater than 2 X 106 Units/mg. CHEMICON Cat. No. GF003)
      4. Epidermal growth factor (EGF; Specific Activity greater than 1 x 107 Units/mg; CHEMICON Cat. No. GF001) 5. Laminin (Sigma Cat. No. L-2020)
      5. Accutase (CHEMICON Cat. No. SCR005)
      6. Tissue culture-ware
      7. Phosphate-Buffered Saline (1X PBS) (CHEMICON Cat. No. BSS-1005-B)
      8. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
      9. Blocking Solution (5 percent normal donkey serum, 0.3 % Triton X-100 in 1X PBS)
      10. Primary and secondary antibodies
      References
      Product Information
      PresentationReNcell® NSC Maintenance Medium contains DMEM/F12, HEPES, L-glutamine, human serum albumin, human transferrin, putrescine dihydrochloride, human recombinant insulin, L-thyroxine, Tri-iodo-thyronine, progesterone, sodium selenite, heparin, corticosterone. The media does not contain antibiotics.
      Quality LevelMQ100
      Applications
      ApplicationReNcell® NSC Maintenance Medium is a defined serum-free, growth factor-free neural stem cell medium that has been optimized for the growth & in vitro differentiation of ReNcell® immortalized human neural progenitor cells.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsSCM005, ReNcell® Neural Stem Cell Maintenance Medium should be stored at -20°C until ready to use. Upon thawing, the maintenance medium should be stored at 2-8°C and given a 1-month expiration dating.
      Packaging Information
      Material Size500 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ReNcell® NSC Maintenance Media SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line.
      Song, Y; Subramanian, K; Berberich, MJ; Rodriguez, S; Latorre, IJ; Luria, CM; Everley, R; Albers, MW; Mitchison, TJ; Sorger, PK
      Sci Data  6  190016  2019

      Show Abstract
      30778261 30778261
      A three-dimensional human neural cell culture model of Alzheimer's disease.
      Choi, SH; Kim, YH; Hebisch, M; Sliwinski, C; Lee, S; D'Avanzo, C; Chen, H; Hooli, B; Asselin, C; Muffat, J; Klee, JB; Zhang, C; Wainger, BJ; Peitz, M; Kovacs, DM; Woolf, CJ; Wagner, SL; Tanzi, RE; Kim, DY
      Nature  2014

      Show Abstract
      25307057 25307057
      Transcriptional signature and memory retention of human-induced pluripotent stem cells.
      Marchetto, Maria C N, et al.
      PLoS ONE, 4: e7076 (2009)  2009

      Show Abstract
      19763270 19763270

      Technical Info

      Title
      ReNcell VM Cell Line - A Model of Human Neural Development and Differentiation