Product Information
Applications
Biological Information
Primary TargetRSK2
Primary Target IC<sub>50</sub>12 and 2.5 nM for wild-type and T493M RSK2, respectively
Secondary targetMSK 1, 2 , 3
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

RSK2 Inhibitor, RMM-46 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Miller, R.M., et al. 2013. J. Am. Chem. Soc. 135, 5298.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-October-2013 JSW
Synonyms(E)-2-Cyano-N-(1-hydroxy-2-methylpropan-2-yl)-3-(3-(3,4,5-trimethoxyphenyl)-1H-indazol-5-yl)acrylamide, RMM46, RSK Inhibitor IV
DescriptionA cell-permeable cyanoacrylamide compound that acts as a potent, reversible, and selective inhibitor of ribosomal S6 kinase 2 (RSK2; IC50 = 12 and 2.5 nM for wild-type and T493M RSK2, respectively). Shown to act by covalently modifying Cys436 at the ATP-binding site. Exhibits good selectivity over NEK2 and PLK1 (IC50 = 530 nM and 2.2 µM) and does not affect the activity of a panel of 26 other protein kinases. Also shown to block phosphorylation of mitogen and stress activated kinases (MSK) 1, 2, and 3 in PMA-stimulated COS7 cells (IC50 ~100 nM) and CREB phosphorylation in PMA- or UV-stimulated HeLa cells (IC50 ~ 300 nM).
FormYellow solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₄H₂₆N₄O₅
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (50 mg/ml). Use only fresh DMSO for reconstitution.
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesMiller, R.M., et al. 2013. J. Am. Chem. Soc. 135, 5298.

Related Products & Applications

Categories

Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Protein Phosphorylation / Dephosphorylation > S6K Inhibitors