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540222 Puromycin, Dihydrochloride - CAS 58-58-2 - Calbiochem

540222
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Overview

Replacement Information

Key Specifications Table

Empirical FormulaCAS #
C₂₂H₂₉N₇O₅ · 2HCl 58-58-2

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540222-100MG
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      Plastic ampoule 100 mg
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      540222-25MG
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          Description
          OverviewProtein synthesis inhibitor. Inhibits translation by causing premature release of nascent polypeptide chains. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
          Catalogue Number540222
          Brand Family Calbiochem®
          Synonyms3ʹ-(α-Amino-p-methoxyhydrocinnamamido)-3ʹ-deoxy-N,N-dimethyladenosine, 2HCl
          References
          ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
          Constam, D.B., et al. 1995. J. Biol. Chem. 270, 26931.
          Wirth, M., et al. 1994. J. Virol. 68, 566.
          Claeyssens, S., et al. 1993. FEBS Lett. 315, 7.
          Hechler, U., et al. 1993. Biochem. Biophys. Res. Commun. 194, 1305.
          Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
          de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
          Kalpaxis, D.L. and Drainas, D. 1992. Arch. Biochem. Biophys. 300, 629.
          Shipston, M.J. and Antoni, F.A. 1992. Biochem. Biophys. Res. Commun. 189, 1382.
          Product Information
          CAS number58-58-2
          FormWhite to off-white solid
          Hill FormulaC₂₂H₂₉N₇O₅ · 2HCl
          Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          Biological Information
          Primary Targetprotein synthesis
          Purity≥98% by TLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSAU7355000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage -20°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Puromycin, Dihydrochloride - CAS 58-58-2 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Puromycin, Dihydrochloride - CAS 58-58-2 - Calbiochem Certificates of Analysis

          TitleLot Number
          540222

          References

          Reference overview
          Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
          Constam, D.B., et al. 1995. J. Biol. Chem. 270, 26931.
          Wirth, M., et al. 1994. J. Virol. 68, 566.
          Claeyssens, S., et al. 1993. FEBS Lett. 315, 7.
          Hechler, U., et al. 1993. Biochem. Biophys. Res. Commun. 194, 1305.
          Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
          de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
          Kalpaxis, D.L. and Drainas, D. 1992. Arch. Biochem. Biophys. 300, 629.
          Shipston, M.J. and Antoni, F.A. 1992. Biochem. Biophys. Res. Commun. 189, 1382.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision16-May-2008 RFH
          Synonyms3ʹ-(α-Amino-p-methoxyhydrocinnamamido)-3ʹ-deoxy-N,N-dimethyladenosine, 2HCl
          DescriptionProkaryotic and eukaryotic protein synthesis inhibitor. Nucleoside that resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the "A site" of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Used for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product, puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Like cycloheximide, puromycin is also used as a general protein synthesis inhibitor in numerous cellular assays.
          FormWhite to off-white solid
          CAS number58-58-2
          RTECSAU7355000
          Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
          Structure formulaStructure formula
          Purity≥98% by TLC
          SolubilityH₂O (50 mg/ml) and Methanol (20 mg/ml)
          Storage -20°C
          Protect from moisture
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Harmful
          Merck USA index14, 7943
          ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
          Constam, D.B., et al. 1995. J. Biol. Chem. 270, 26931.
          Wirth, M., et al. 1994. J. Virol. 68, 566.
          Claeyssens, S., et al. 1993. FEBS Lett. 315, 7.
          Hechler, U., et al. 1993. Biochem. Biophys. Res. Commun. 194, 1305.
          Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
          de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
          Kalpaxis, D.L. and Drainas, D. 1992. Arch. Biochem. Biophys. 300, 629.
          Shipston, M.J. and Antoni, F.A. 1992. Biochem. Biophys. Res. Commun. 189, 1382.