72635 PureGenome™ Tissue DNA Extraction Kit

72635
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72635-1KIT
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      Description
      Overview

      Product Description

      The PureGenome™ Tissue DNA Extraction Kit is a simple procedure for the rapid isolation of total DNA (e.g. genomic, mitochondrial, parasitic, microbial, viral) from a variety of soft and solid tissues, cells and a range of biological liquids. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is also compatible with buffy coat, bone marrow, cells from culture, whole blood (fresh or stored), serum, plasma, and many biological liquid samples. For processing, simply digest the sample with the supplied Proteinase K, then add the specially formulated Genomic Lysis Buffer, vortex, and transfer the mixture(s) to a supplied column. The product features Fast-Spin column technology to yield high-quality, purified DNA (see Figure 1 and Figure 2 below). Enzyme inhibitors are effectively removed during the purification process. DNA purified using the PureGenome™ Tissue DNA Extraction Kit is ideally suited for Next Generation Sequencing. In addition, DNA purified using this kit can be used for Sanger-chemistry sequencing, PCR, Southern blotting, endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.

      This kit is capable of recovering genomic and mitochondrial DNA sized fragments from 100 bp to ≥ 40 kb. If present, parasitic, microbial, and viral DNA will also be recovered. Typical fragment sizes range from 25–35 kb.

      One kit is sufficient for 50 test samples.


      Panel Components

      • 5 mg       PureGenome Proteinase K
      • 0.5 ml     PureGenome Proteinase K Storage Buffer
      • 5 ml        PureGenome 2X Digestion Buffer
      • 50 ml      PureGenome Genomic Lysis Buffer
      • 15 ml      PureGenome DNA-Pre-Wash Buffer
      • 50 ml       PureGenome g-DNA Wash Buffer
      • 10 ml       PureGenome DNA Elution Buffer
      • 1 EA         PureGenome SpinIIC Columns (50 tubes/EA)
      • 1 EA        PureGenome Collection Tubes (100 tubes/EA)


      Storage: Store all components at room temperature prior to initial use. This kit is stable for at least one year at the recommended storage temperature. Reagents are routinely tested on lot-to-lot basis to ensure they provide maximal performance and reliability.

      Catalogue Number72635
      Brand Family Novagen®
      Application Data
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 11-20/21/22-32-36/37/38-42-43-67

      Highly flammable.
      Harmful by inhalation, in contact with skin and if swallowed.
      Contact with acids liberates very toxic gas.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation.
      May cause sensitization by skin contact.
      Vapors may cause drowsiness and dizziness.
      S PhraseS: 16-22-24/25-26-36/37/39-45-7

      Keep away from sources of ignition - No Smoking.
      Do not breathe dust.
      Avoid contact with skin and eyes.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Keep container tightly closed.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications