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Description
Overview
A convenient and highly reproducible kit for the precipitation and clean-up of proteins. Yield of precipitated proteins is higher than that of traditional methods with improved solubility of the precipitated protein. Contains sufficient components to precipitate 200 samples of ≤200 µl. Requires 150 ml of ethanol (not provided). Kit delivers protein solutions of very low conductivity.
Catalogue Number
539180
Brand Family
Calbiochem®
Application Data
The ProteoExtract® Protein Precipitation Kit yields protein samples with significantly lower conductivity then other precipitation methods.
Proteins at a concentration of 1 mg/ml were precipitated from 1 M sodium chloride solutions according to the Detailed Protocol. The same experiment was performed using standard protocols as TCA/DOC or acetone precipitation. Protein pellets were solubilized in IEF buffer for conductivity measurement.
Materials Required but Not Delivered
• Ethanol • Microcentrifuge • Micropipettes and tips, 10 µl, 200 µl and 1 ml size
References
Product Information
Form
200 Precipitations
Kit contains
Precipitant 1, Precipitant 2, Precipitant 3, Precipitant 4, Wash Solution, and a user protocol.
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Intended use
The ProteoExtract® Protein Precipitation Kit is designed for the concentration and clean-up of proteins from aqueous samples.
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
+15°C to +30°C
Storage Conditions
Upon arrival, all components of the kit can be stored at room temperature (20°C). For ease of use, the prepared Precipitation Agent and the Wash Solution can be stored at -20°C (see Reagent Preparation).
Do not freeze
Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit contains
Precipitant 1, Precipitant 2, Precipitant 3, Precipitant 4, Wash Solution, and a user protocol.
