70663 MilliporeProteinase K, Lyophilized

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.

Proteinase K, Lyophilized MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
User Protocol
Citations

Recommended Products

Overview

Replacement Information

Pricing & Availability

Catalog Number	Availability	Packaging	Qty/Pack	Price	Quantity
70663-4	Retrieving availability... — Fulfilment and delivery delayed Stocked Discontinued Limited Quantities Available Available Login to See Inventory Stocked Fulfilment and delivery delayed Stocked Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic ampoule	100 mg	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
70663-5	Retrieving availability... — Fulfilment and delivery delayed Stocked Discontinued Limited Quantities Available Available Login to See Inventory Stocked Fulfilment and delivery delayed Stocked Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Glass bottle	500 mg	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites

Description
Overview	Proteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/ml for protein removal and enzyme inactivation, and up to 2 mg/ml for tissue treatment. The Proteinase K, Lyophilized powder can be prepared as a 20 mg/ml stock solution in water and stored in aliquots at -20°C. The enzyme is also available as a ready-to-use concentrated stock solution (600 mAU/ml) that is convenient for routine use in most applications. 1 mg of Proteinase K is the equivalent of 30 mAU (AU = Anson unit). The Novagen Proteinase K products are free of detectable DNase and RNase
Catalogue Number	70663
Brand Family	Novagen®

References

Product Information
CAS number	39450-01-6
Unit of Definition	One AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C.

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 36/37/38-42 Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation.
S Phrase	S: 22-24-26-36/37 Do not breathe dust. Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves.

Product Usage Statements

Storage and Shipping Information
Ship Code	Shipped with Blue Ice or with Dry Ice
Toxicity	Harmful
Storage	+2°C to +8°C
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

Specifications

Documentation

Proteinase K, Lyophilized SDS

Title
Safety Data Sheet (SDS)

Proteinase K, Lyophilized Certificates of Analysis

Title	Lot Number
70663	Enter Lot Number

Citations

Title

Shannon M. Anderson, et al. (2007) New markers for murine memory B cells that define mutated and unmutated subsets. Journal of Experimental Medicine 204, 2103-2114.

Andrew A. Horwitz, et al. (2007) ATP-induced structural transitions in PAN, the proteasome-regulatory ATPase complex in Archaea. Journal of Biological Chemistry 282, 22921-22929.

User Protocols

Title
TB271 Proteinase K

Related Products

Catalog Number

Description

71049

Proteinase K Solution, 600 mAU/ml

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