IP02 Protein A Agarose Suspension

IP02
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IP02-1.5ML
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      Plastic ampoule 1.5 ml
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      Description
      OverviewDesigned for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG2a and IgG2b and rabbit IgG.
      Catalogue NumberIP02
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid slurry
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoprecipitation
      Application CommentsAgarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG2a or IgG2b monoclonal antibodies. Mouse IgG1 or IgG3 and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protein A Agarose Suspension SDS

      Title

      Safety Data Sheet (SDS) 

      Protein A Agarose Suspension Certificates of Analysis

      TitleLot Number
      IP02
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-September-2007 RFH
      DescriptionProtein A conjugated to agarose.
      FormLiquid slurry
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      CommentsAgarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG2a or IgG2b monoclonal antibodies. Mouse IgG1 or IgG3 and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
      Life Science Research > Protein Sample Preparation > Protein Purification > Agarose Bead Affinity Purification > Agarose Beads for IP & Antibody Purification