AP1062 PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb

AP1062
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, Pm, R Rb Polyclonal Antibody

Pricing & Availability

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AP1062-50UL
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      AP1062-5MG
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          Description
          OverviewRecognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293 in HEK293 cells.
          Catalogue NumberAP1062
          Brand Family Calbiochem®
          SynonymsAnti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb
          Application Data
          Detection of human PDH-E3α, phosphorylated at Ser293, by immunocytochemistry. Samples: HEK293 cells treated with DCA (bottom panels) or left untreated (top panels) and subsequently fixed with 4% paraformaldehyde. Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:500). Detection: fluorescence (red) using DAPI to counterstain the nuclei (blue) and AlexaFluor®-Phalloidin to counterstain actin filaments (green).

          Detection of human PDH-E1α, phosphorylated at Ser293, by immunoprecipitation, followed by immunoblotting. Samples: Whole cell lysate (375 µg) from HEK293 cells. Antibody for IP: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:50). . Antibodyfor IB: Anti-PDH-E1α (pSer293 Rabbit pAb (Cat. No. AP1062) (1:1000) Detection: chemiluminescence.

          Detection of human PDH-E1α, phosphorylated at Ser293, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) and HEK293 cells treated with dichloroacetate. Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:10,000). Detection: chemiluminescence.
          References
          ReferencesSeifert, F., et al. 2007. Biochemistry 21, 6277.
          Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
          Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
          Positive controlHEK293 cells
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Application NotesImmunoblotting (1:10,000)
          Immunoprecipitation (1:50)
          Immunocytochemistry (1:500)
          Application CommentsAntibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Primate
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb Certificates of Analysis

          TitleLot Number
          AP1062

          References

          Reference overview
          Seifert, F., et al. 2007. Biochemistry 21, 6277.
          Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
          Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

          Citations

          Title
          Madhok, B.M., et al. 2010. British Journal of Cancer 102, 1746.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision30-August-2011 JSW
          SynonymsAnti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb
          ApplicationImmunoblotting (1:10,000)
          Immunoprecipitation (1:50)
          Immunocytochemistry (1:500)
          Application Data
          Detection of human PDH-E3α, phosphorylated at Ser293, by immunocytochemistry. Samples: HEK293 cells treated with DCA (bottom panels) or left untreated (top panels) and subsequently fixed with 4% paraformaldehyde. Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:500). Detection: fluorescence (red) using DAPI to counterstain the nuclei (blue) and AlexaFluor®-Phalloidin to counterstain actin filaments (green).

          Detection of human PDH-E1α, phosphorylated at Ser293, by immunoprecipitation, followed by immunoblotting. Samples: Whole cell lysate (375 µg) from HEK293 cells. Antibody for IP: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:50). . Antibodyfor IB: Anti-PDH-E1α (pSer293 Rabbit pAb (Cat. No. AP1062) (1:1000) Detection: chemiluminescence.

          Detection of human PDH-E1α, phosphorylated at Ser293, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) and HEK293 cells treated with dichloroacetate. Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:10,000). Detection: chemiluminescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293.
          BackgroundPyruvate dehydogenase (PDH) complex (PDC) catalyzes the irreversible conversion of pyruvate to acetyl CoA, providing the link between glycolyis and the TCA cycle. Dysregulation of the PDC in skeletal muscle has been implicated in type 2 diabetes and mutations in the PDC result in various mitochondrial diseases. The PDC is a multienzyme complex composed of several copies of three catalytic proteins (E1, E2, and E3): one structural (E3-binding protein) and two regulatory proteins (PDH kinase [PDK] and PDH phosphatase [PDP]). The activity of the PDC complex is mainly regulated by phosphorylation-dehosphorylation of three specific serine residues (232, 293, 300) on the E1 subunit. Phosphorylation of PDH determines the activity of the complex. Phosphorylation by one of four PDK isoforms inactivates the complex, while removal of the phosphate by one of two PDP isoforms activates the complex. The activity of the PDP1 isoform is sensitive to Ca2+, while the PDK isoforms are regulated by the reduction and acetylation status of the E2 subunit, pyruvate, and the small molecule the anti cancer dichloroacetate. (DCA).
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α
          IsotypeIgG
          Specieshuman, mouse, primate, rat
          Positive controlHEK293 cells
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
          Preservative≤0.1% sodium azide
          CommentsAntibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Toxicity Standard Handling
          ReferencesSeifert, F., et al. 2007. Biochemistry 21, 6277.
          Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
          Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
          CitationMadhok, B.M., et al. 2010. British Journal of Cancer 102, 1746.