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PK1008 PhosphoDetect™ Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb

PK1008
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

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PK1008-50UL
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      Description
      OverviewRecognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751 in PDGF-stimulated L6, HepG2, and NIH3T3 cells.
      Catalogue NumberPK1008
      Brand Family Calbiochem®
      SynonymsAnti-Platelet Derived Growth Factor Receptor-β, Phospho-Specific (Tyr⁷⁵¹)
      Application Data
      Detection of mouse and human PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Samples: Whole cell lysate from L6, HepG2, and NIH3T3 cells left untreated (-) or treated with PDGF (40 ng/ml for 5 min; +). Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1007) (1:1000). Detection: chemiluminescence.

      Detection of human PDGFR-β, phosphorylated on Tyr751, by staining paraffin sections. Sample: Breast carcinoma tissue. Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:50). Detection: DAB with hematoxylin counterstain.

      Detection of mouse PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Sample: NIH3T3 cells left untreated (blue) or treated with PDGF (green). Primary antibodies: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:200) and irrelevant control antibody (red). Detection: fluorescence.
      References
      ReferencesBetsholtz, C., et al. 2001. Bioessays 23, 494.
      Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res. 80, 1.
      Ramalingam, K., et al. 1995. Bioorg. Med. Chem. 3, 1263.
      Panyotou, G. et al. 1992. EMBO J. 11, 4261.
      Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res. 266, 39.
      Deuel, T.F., et al. 1988. Biofactors 1, 213.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Positive controlBreast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Paraffin Sections
      Flow Cytometry
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesFlow Cytometry (1:200)
      Immunoblotting (1:1000)
      Immunoprecipitation (1:50)
      Paraffin Sections (1:50, heat pre-treatment required; see comments)
      Application CommentsFor staining paraffin sections bring slides to a boil in 1 mM EDTA, pH 8.0 and continue to heat at sub-boiling temperature for 15 min; no cooling is necessary. Wash paraffin sections with 1X TBS, 0.1% Tween®-20 detergent. For immunoblotting membranes should be incubated with antibody diluted in 1X TBS, 5% BSA, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁷⁵¹ phosphorylation site of human PDGF receptor-β, coupled to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PK1008

      References

      Reference overview
      Betsholtz, C., et al. 2001. Bioessays 23, 494.
      Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res. 80, 1.
      Ramalingam, K., et al. 1995. Bioorg. Med. Chem. 3, 1263.
      Panyotou, G. et al. 1992. EMBO J. 11, 4261.
      Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res. 266, 39.
      Deuel, T.F., et al. 1988. Biofactors 1, 213.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      SynonymsAnti-Platelet Derived Growth Factor Receptor-β, Phospho-Specific (Tyr⁷⁵¹)
      ApplicationFlow Cytometry (1:200)
      Immunoblotting (1:1000)
      Immunoprecipitation (1:50)
      Paraffin Sections (1:50, heat pre-treatment required; see comments)
      Application Data
      Detection of mouse and human PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Samples: Whole cell lysate from L6, HepG2, and NIH3T3 cells left untreated (-) or treated with PDGF (40 ng/ml for 5 min; +). Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1007) (1:1000). Detection: chemiluminescence.

      Detection of human PDGFR-β, phosphorylated on Tyr751, by staining paraffin sections. Sample: Breast carcinoma tissue. Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:50). Detection: DAB with hematoxylin counterstain.

      Detection of mouse PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Sample: NIH3T3 cells left untreated (blue) or treated with PDGF (green). Primary antibodies: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:200) and irrelevant control antibody (red). Detection: fluorescence.
      DescriptionRabbit polyclonal antibody purified by protein A and immunoaffinity chromatography. Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751.
      BackgroundThe PDGF family of growth factors consists of several disulfide-linked dimeric isoforms-PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC, and PDGF-DD-that bind in distinct patterns to the specific receptor tyrosine kinases, PDGFR-α and PDGFR-β. PDGFR-α can bind to all isoforms of PDGF except PDGF-DD and PDGFR-β only binds to PDGF-DD. Both receptors can form homo- and heterodimers, including heterodimer formation with EGFR, which can also be stimulated by PDGF. Similar to other receptor tyrosine kinases, ligand binding leads to receptor dimerization and autophosphorylation, which permits binding and activatino of cytoplasmic SH2 domain-containing signal transduction molecules, including Grb2, Src, GAP, PI-3K, PLCγ, and Nck. As a result, a number of different signaling pathways are initiated leading to cell growth, actin reorganization, cell migration, and cell differentiation. Tyr751 of PDGFR-β is the docking site for PI-3K. Phosphorylated pentapeptides derived from Tyr751 are reported to inhibit the association of PI-3K with PDGFR-β.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁷⁵¹ phosphorylation site of human PDGF receptor-β, coupled to KLH
      IsotypeIgG1
      Specieshuman, mouse, rat
      Positive controlBreast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      CommentsFor staining paraffin sections bring slides to a boil in 1 mM EDTA, pH 8.0 and continue to heat at sub-boiling temperature for 15 min; no cooling is necessary. Wash paraffin sections with 1X TBS, 0.1% Tween®-20 detergent. For immunoblotting membranes should be incubated with antibody diluted in 1X TBS, 5% BSA, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Toxicity Standard Handling
      ReferencesBetsholtz, C., et al. 2001. Bioessays 23, 494.
      Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res. 80, 1.
      Ramalingam, K., et al. 1995. Bioorg. Med. Chem. 3, 1263.
      Panyotou, G. et al. 1992. EMBO J. 11, 4261.
      Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res. 266, 39.
      Deuel, T.F., et al. 1988. Biofactors 1, 213.