442685 PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb

442685
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, R Rb Polyclonal Antibody

Pricing & Availability

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442685-100UL
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      Plastic ampoule 100 ul
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      Description
      OverviewRecognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204.
      Catalogue Number442685
      Brand Family Calbiochem®
      Application Data
      Detection of human MAP Kinase ERK1/ERK2, phosphorylated on Thr202/Tyr204, by immunoblotting. Samples: Whole cell lysate from human T47D cells untreated (lane 1) or treated with lambda-phosphatase (lane 2). Primary antibody: PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr202/Tyr204) Rabbit pAb (Cat. No. 442685) (1:1000). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 μg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Dot Blot
      Immunoblotting (Western Blotting)
      Application NotesDot Blot (1:1000)
      Immunoblotting (1:1000)
      Application CommentsDoes not react with the unphosphorylated proteins. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide surrounding the Thr²⁰²/Tyr²⁰⁴ phosphorylation sites of human ERK1/ERK2
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Rabbit pAb Certificates of Analysis

      TitleLot Number
      442685
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2007 RFH
      ApplicationDot Blot (1:1000)
      Immunoblotting (1:1000)
      Application Data
      Detection of human MAP Kinase ERK1/ERK2, phosphorylated on Thr202/Tyr204, by immunoblotting. Samples: Whole cell lysate from human T47D cells untreated (lane 1) or treated with lambda-phosphatase (lane 2). Primary antibody: PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr202/Tyr204) Rabbit pAb (Cat. No. 442685) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide surrounding the Thr²⁰²/Tyr²⁰⁴ phosphorylation sites of human ERK1/ERK2
      IsotypeIgG
      Specieshuman, rat
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 μg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      CommentsDoes not react with the unphosphorylated proteins. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling

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