341292 PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb

341292
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Ch, Dr, Fg, H, M, R Rb Polyclonal Antibody

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341292-10T
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      Description
      OverviewRecognizes the ~125 kDa FAK protein when phosphorylated at Tyr397. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
      Catalogue Number341292
      Brand Family Calbiochem®
      SynonymsAnti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)
      Application Data
      Detection of FAK, phosphorylated on Tyr397, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr397) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      References
      ReferencesFornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235.
      Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250.
      Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
      Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758.
      Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (no Mg²+ and Ca²+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Positive controlChicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesImmunoblotting (1:1000)
      Immunohistochemistry (see comments; heat pre-treatment required)
      Application CommentsAlthough the antibody was adsorbed against a phospho-peptide corresponding to Pyk2, some cross-reactivity may still be detected in cases where Pyk2 is overexpressed relative to FAK within the same cell system. This antibody has also been reported to work for immunohistochemistry; heat pre-treatment with citrate buffer, pH 6.0 is required. When diluted 1:1000 there is sufficient antibody for 10 miniblots at 10 ml per blot. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁹⁷ phosphorylation site of human FAK
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Drosophila
      • Frog
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb Certificates of Analysis

      TitleLot Number
      341292

      References

      Reference overview
      Fornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235.
      Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250.
      Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
      Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758.
      Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-November-2008 JSW
      SynonymsAnti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)
      ApplicationImmunoblotting (1:1000)
      Immunohistochemistry (see comments; heat pre-treatment required)
      Application Data
      Detection of FAK, phosphorylated on Tyr397, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr397) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated on Tyr397.
      BackgroundDiscovered as a substrate for Src, FAK acts as a key element of integrin signaling. FAK is a non-receptor tyrosine kinase that plays a central role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. The Tyr397 autophosphorylation site binds Src family SH2 and the p85 subunit of PI3-Kinase.
      HostRabbit
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁹⁷ phosphorylation site of human FAK
      IsotypeIgG
      SpeciesDrosophila, chicken, frog, human, mouse, rat
      Positive controlChicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF.
      FormLiquid
      FormulationIn Dulbecco's PBS (no Mg²+ and Ca²+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Preservative≤0.1% sodium azide
      CommentsAlthough the antibody was adsorbed against a phospho-peptide corresponding to Pyk2, some cross-reactivity may still be detected in cases where Pyk2 is overexpressed relative to FAK within the same cell system. This antibody has also been reported to work for immunohistochemistry; heat pre-treatment with citrate buffer, pH 6.0 is required. When diluted 1:1000 there is sufficient antibody for 10 miniblots at 10 ml per blot. Antibody should be titrated for optimal results in individual systems.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesFornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235.
      Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250.
      Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
      Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758.
      Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.

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