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DR1025 PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb

DR1025
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, Mk, M, R Rb Polyclonal Antibody

Pricing & Availability

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DR1025-50UL
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      Plastic ampoule 50 ul
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      Description
      OverviewRecognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells.
      Catalogue NumberDR1025
      Brand Family Calbiochem®
      Application Data
      Detection of human and monkey Chk1, phosphorylated at Ser317, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer317) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.
      References
      ReferencesZhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129.
      Shieh, S.Y., et al. 2000. Genes Dev. 14, 289.
      Martinho, R.G., et al. 1998. EMBO J. 17, 7239.
      Zeng, Y., et al. 1998. Nature 395, 507.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Positive controlLysates from COS cells treated with UV
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesImmunoblotting (1:1000)
      Immunocytochemistry (1:500, fluorescence)
      Paraffin Sections (1:50)
      Application CommentsDoes not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Monkey
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb Certificates of Analysis

      TitleLot Number
      DR1025

      References

      Reference overview
      Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129.
      Shieh, S.Y., et al. 2000. Genes Dev. 14, 289.
      Martinho, R.G., et al. 1998. EMBO J. 17, 7239.
      Zeng, Y., et al. 1998. Nature 395, 507.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2007 RFH
      ApplicationImmunoblotting (1:1000)
      Immunocytochemistry (1:500, fluorescence)
      Paraffin Sections (1:50)
      Application Data
      Detection of human and monkey Chk1, phosphorylated at Ser317, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer317) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser317.
      BackgroundChk1 is a downstream protein kinase of ATM/ATR that plays an improtant role in DNA damage checkpoint, development, and tumor suppression. Chk1 is activated in response to various forms of genotoxic stress and replication blocks. Chk1 is activated by phosphorylation on Ser317 and Ser345. Chk1 can inactivate cdc25c and phosphorylate p53.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
      IsotypeIgG
      Specieshuman, monkey, mouse, rat
      Positive controlLysates from COS cells treated with UV
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      CommentsDoes not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Toxicity Standard Handling
      ReferencesZhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129.
      Shieh, S.Y., et al. 2000. Genes Dev. 14, 289.
      Martinho, R.G., et al. 1998. EMBO J. 17, 7239.
      Zeng, Y., et al. 1998. Nature 395, 507.