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516562 Pertussis Toxin, Salt-Free, Bordetella pertussis - CAS 70323-44-3 - Calbiochem

516562
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CAS #
70323-44-3

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516562-50UG
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      Description
      OverviewA protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins. The holotoxin has five dissimilar subunits S-1, S-2, S-3, S-4, and S-5, in a molar ratio of 1:1:1:2:1, S-1 (A protomer) is responsible for the enzymatic activity of the toxin, and S-2, S-3, S-4, and S-5 form the B oligomer which is responsible for the toxins binding to the cell surface. NOTE: This product is not activated, hence for use in cell-free systems, activation is required.
      Catalogue Number516562
      Brand Family Calbiochem®
      SynonymsIslet-activating Protein
      References
      ReferencesFryer, M.W. 1992. Neurosci. Lett. 146, 84.
      Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
      Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
      Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
      Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684.
      Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
      Kaslow, H.R., et al. 1987. Biochemistry 26, 123.
      Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
      Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
      Product Information
      CAS number70323-44-3
      ATP CompetitiveN
      FormSalt-free lyophilized solid
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activity≤0.01 ng/ml. The activity is defined as the lowest concentration that promotes a clustering pattern in the CHO cell assay
      Primary TargetADP-ribosylation of guanine nucleotide-binding regulatory proteins
      PurityFive distinct bands by SDS-PAGE
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSXW5883750
      Safety Information
      R PhraseR: 23/24/25

      Toxic by inhalation, in contact with skin and if swallowed.
      S PhraseS: 36/37/39-45

      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Toxic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Do not freeze Yes
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). For long term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for up to 6 months for use in CHO cell assays and for up to 2 months for use in non-cellular systems.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Pertussis Toxin, Salt-Free, Bordetella pertussis - CAS 70323-44-3 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Pertussis Toxin, Salt-Free, Bordetella pertussis - CAS 70323-44-3 - Calbiochem Certificates of Analysis

      TitleLot Number
      516562

      References

      Reference overview
      Fryer, M.W. 1992. Neurosci. Lett. 146, 84.
      Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
      Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
      Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
      Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684.
      Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
      Kaslow, H.R., et al. 1987. Biochemistry 26, 123.
      Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
      Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-August-2008 RFH
      SynonymsIslet-activating Protein
      DescriptionA protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and GT. Affects glucose homeostasis, immune cell function, and the induction of leukocytosis. Used in the study of adenylate cyclase regulation and the role of Gi proteins. The holotoxin has five dissimilar subunits S-1, S-2, S-3, S-4, and S-5, in a molar ratio of 1:1:1:2:1. S-1 (a protomer) is responsisble for the enzymatic activity of the toxin, and other subunits (2-5) form the B oligomer which is responsible for the toxin's binding to the cell surface. Note: this product is not activated. If used with an intact cell system, the cells will activate the toxin. If used in a cell-free system, activation may be achieved by the methods of either Kaslow and Burns or Moss (see references).
      FormSalt-free lyophilized solid
      CAS number70323-44-3
      RTECSXW5883750
      PurityFive distinct bands by SDS-PAGE
      Biological activity≤0.01 ng/ml. The activity is defined as the lowest concentration that promotes a clustering pattern in the CHO cell assay
      SolubilityReconstitute with 500 µl sterile H₂O or aqueous buffer. Obtain a uniform suspension before each use by scraping the walls of the tube and gently pipetting the solution to mix.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). For long term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for up to 6 months for use in CHO cell assays and for up to 2 months for use in non-cellular systems.
      Toxicity Toxic
      ReferencesFryer, M.W. 1992. Neurosci. Lett. 146, 84.
      Gierschik, P. 1992. Curr. Topics Microbiol. Immunol. 175, 69.
      Harada, J., et al. 1992. Eur. J. Pharmacol. 227, 301.
      Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem. 267, 13735.
      Kaslow, H.R., and Burns, D.L. 1992. FASEB J. 6, 2684.
      Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap. 263, 1479.
      Kaslow, H.R., et al. 1987. Biochemistry 26, 123.
      Hewlett, E.L., et al. 1983. Infect. Immun. 40, 1198.
      Moss, J., et al. 1983. J. Biol. Chem. 258, 11879.

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      Categories

      Life Science Research > Inhibitors and Biochemicals > Biochemicals > Toxins